Detailed information of TA system TA063889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 467028..467665
• Replicon: chromosome
• Accession: NZ_CP015911
• Organism: Bacillus velezensis strain LS69

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: A8142_RS02335
• Protein ID: WP_003156187.1
• Coordinates: 467315..467665 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: A8142_RS02330
• Protein ID: WP_003156188.1
• Coordinates: 467028..467309 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8142_RS02310	463393..463992	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
A8142_RS02315	464085..464450	+	366	WP_007609580.1	holo-ACP synthase	-
A8142_RS02320	464615..465622	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
A8142_RS02325	465739..466908	+	1170	WP_032873001.1	alanine racemase	-
A8142_RS02330	467028..467309	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
A8142_RS02335	467315..467665	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
A8142_RS02340	467783..468604	+	822	WP_003156182.1	STAS domain-containing protein	-
A8142_RS02345	468609..468974	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
A8142_RS02350	468977..469378	+	402	WP_003156178.1	anti-sigma regulatory factor	-
A8142_RS02355	469390..470397	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
A8142_RS02360	470461..470790	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
A8142_RS02365	470787..471269	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
A8142_RS02370	471235..472023	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
A8142_RS02375	472023..472625	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T63889 WP_003156187.1 NZ_CP015911:467315-467665 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T63889 NZ_CP015911:467315-467665 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT63889 WP_003156188.1 NZ_CP015911:467028-467309 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT63889 NZ_CP015911:467028-467309 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5