Detailed information of TA system TA063881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 117243..117814
• Replicon: plasmid Efsorialis-p1
• Accession: NZ_CP015884
• Organism: Enterococcus faecalis strain sorialis

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A6B47_RS14905
• Protein ID: WP_087548803.1
• Coordinates: 117243..117584 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6B1XRC3
• Locus tag: A6B47_RS14910
• Protein ID: WP_000958277.1
• Coordinates: 117584..117814 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6B47_RS14890	114338..115135	-	798	Protein_118	cell wall hydrolase	-
A6B47_RS14895	115345..115947	-	603	WP_010821245.1	Fic family protein	-
A6B47_RS14900	116193..117131	-	939	WP_087548801.1	hypothetical protein	-
A6B47_RS14905	117243..117584	-	342	WP_087548803.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A6B47_RS14910	117584..117814	-	231	WP_000958277.1	hypothetical protein	Antitoxin
A6B47_RS14915	118018..118638	+	621	WP_168354168.1	recombinase family protein	-
A6B47_RS14920	118677..118877	+	201	WP_087548804.1	hypothetical protein	-
A6B47_RS14925	119044..119244	+	201	WP_077933125.1	transcriptional regulator	-
A6B47_RS14935	119557..119703	+	147	WP_077933127.1	class II bacteriocin	-
A6B47_RS14940	119720..120004	+	285	WP_077933126.1	bacteriocin immunity protein	-
A6B47_RS14945	120028..120300	+	273	WP_087548805.1	bacteriocin immunity protein	-
A6B47_RS15465	120964..121142	-	179	Protein_129	hypothetical protein	-
A6B47_RS14955	121684..121935	+	252	WP_077933104.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	prgB/asc10 / cap8E / ugd	1..125418	125418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13289.54 Da
• Isoelectric Point: 9.3985

>T63881 WP_087548803.1 NZ_CP015884:c117584-117243 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLRQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T63881 NZ_CP015884:c117584-117243 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGACAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8536.45 Da
• Isoelectric Point: 4.2412

>AT63881 WP_000958277.1 NZ_CP015884:c117814-117584 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT63881 NZ_CP015884:c117814-117584 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6B1XRC3