Detailed information of TA system TA063851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1087577..1088277
• Replicon: plasmid pCasidaAA
• Accession: NZ_CP015881
• Organism: Ensifer adhaerens strain Casida A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FA04_RS24635
• Protein ID: WP_034800191.1
• Coordinates: 1087840..1088277 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1H0BN26
• Locus tag: FA04_RS24630
• Protein ID: WP_034800189.1
• Coordinates: 1087577..1087843 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA04_RS24615	1083916..1084965	+	1050	WP_034800187.1	ABC transporter permease	-
FA04_RS24620	1084967..1085908	+	942	WP_034800188.1	ABC transporter permease	-
FA04_RS24625	1085905..1087196	+	1292	Protein_972	amidohydrolase family protein	-
FA04_RS24630	1087577..1087843	+	267	WP_034800189.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FA04_RS24635	1087840..1088277	+	438	WP_034800191.1	type II toxin-antitoxin system VapC family toxin	Toxin
FA04_RS24640	1088554..1088802	+	249	WP_034800193.1	hypothetical protein	-
FA04_RS24645	1089019..1090059	+	1041	WP_034800195.1	ABC transporter substrate-binding protein	-
FA04_RS24650	1090185..1090949	+	765	WP_060529703.1	ABC transporter permease	-
FA04_RS24655	1090965..1091768	+	804	WP_034800199.1	ABC transporter permease	-
FA04_RS24660	1091765..1092853	+	1089	WP_034800202.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	katA	1..1736943	1736943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-124)

Antitoxin

• SpoVT-AbrB domain (17-54)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15304.68 Da
• Isoelectric Point: 6.9280

>T63851 WP_034800191.1 NZ_CP015881:1087840-1088277 [Ensifer adhaerens]
VSNLFMLDTNIVSELARNPRGQVAERIAAVGSEAICVSIITAAELRYGCAKKGSPKLLAQIEALLESILVLALDVPADAK
YGNIRTELEAAGKPIGPNDLLIAAHACAAGAVLVTANAREFTRIPNLRVENWLDATSKQLRGASN

Nucleotide

• Length: 438 bp

>T63851 NZ_CP015881:1087840-1088277 [Ensifer adhaerens]
GTGAGCAACCTTTTCATGCTGGACACAAATATCGTGTCCGAGTTGGCTCGGAACCCGAGAGGGCAGGTCGCTGAACGTAT
TGCGGCGGTCGGGTCGGAGGCAATCTGCGTCAGCATCATAACGGCGGCAGAATTGCGTTATGGATGCGCCAAGAAGGGTT
CCCCAAAGCTGCTTGCCCAAATCGAAGCCCTTCTAGAAAGCATTCTGGTGCTCGCGCTCGACGTGCCCGCCGACGCCAAG
TACGGCAACATCCGCACTGAGTTGGAAGCTGCAGGCAAACCAATCGGCCCAAATGACCTATTGATCGCTGCTCATGCCTG
TGCCGCAGGCGCAGTCCTCGTCACAGCCAATGCCCGTGAGTTCACGCGCATTCCCAACCTTCGGGTTGAAAACTGGCTGG
ACGCGACCTCAAAGCAACTTAGGGGTGCCTCCAACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.58 Da
• Isoelectric Point: 4.8781

>AT63851 WP_034800189.1 NZ_CP015881:1087577-1087843 [Ensifer adhaerens]
MPQIRHNEPPAREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLIIEPLRRKNLLEVLAGLDPLGPEDQFPDIDETL
LPAKEIDL

Nucleotide

• Length: 267 bp

>AT63851 NZ_CP015881:1087577-1087843 [Ensifer adhaerens]
ATGCCGCAGATTAGACACAACGAACCTCCTGCGAGGGAAGCTAAGCTCTTCCGGAATAACAAGAGCCAGGCTGTTCGCAT
CCCGGCTGACTTTGAGCTTCCTGGCGATCGAGTGATGATCCATCGCGACGGGGATCGGCTCATAATAGAGCCGCTGCGTC
GCAAGAACCTGCTGGAAGTCTTGGCAGGTCTGGATCCTCTTGGGCCTGAAGATCAGTTCCCGGATATTGACGAGACTTTG
TTGCCCGCCAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1H0BN26