Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 750331..750950 Replicon chromosome
Accession NZ_CP015878
Organism Pseudomonas citronellolis strain SJTE-3

Toxin (Protein)


Gene name hicA Uniprot ID W5J3G4
Locus tag A9C11_RS03165 Protein ID WP_009615522.1
Coordinates 750331..750513 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID W5J3H2
Locus tag A9C11_RS03170 Protein ID WP_009615524.1
Coordinates 750546..750950 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C11_RS03155 748938..749096 + 159 WP_009615518.1 YqaE/Pmp3 family membrane protein -
A9C11_RS03160 749384..749872 - 489 WP_064581809.1 phage antirepressor -
A9C11_RS03165 750331..750513 + 183 WP_009615522.1 type II toxin-antitoxin system HicA family toxin Toxin
A9C11_RS03170 750546..750950 + 405 WP_009615524.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A9C11_RS03175 751099..751353 + 255 WP_009615526.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
A9C11_RS03180 751353..751777 + 425 Protein_643 type II toxin-antitoxin system VapC family toxin -
A9C11_RS03185 751782..752942 - 1161 WP_064581810.1 Fic family protein -
A9C11_RS35000 753140..753223 + 84 Protein_645 mRNA interferase -
A9C11_RS03195 753251..753673 + 423 Protein_646 type II toxin-antitoxin system HicB family antitoxin -
A9C11_RS03200 753698..754468 - 771 WP_064581812.1 NERD domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 744647..777320 32673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6962.13 Da        Isoelectric Point: 12.2993

>T63836 WP_009615522.1 NZ_CP015878:750331-750513 [Pseudomonas citronellolis]
MRSREIMELIRADGWFLVEVKGSHHQFRHFTKKGRVTVPHPRSNLPIGTVNSILRQAGLR

Download         Length: 183 bp

>T63836 NZ_CP015878:750331-750513 [Pseudomonas citronellolis]
ATGCGCAGTCGAGAAATCATGGAGTTGATCCGGGCGGATGGCTGGTTCCTGGTGGAGGTGAAAGGCAGTCATCACCAGTT
CCGGCATTTCACCAAGAAGGGGCGGGTGACGGTTCCCCACCCCAGGAGCAACCTGCCGATCGGGACCGTGAACAGTATTC
TGCGCCAGGCCGGCCTGCGATGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14532.23 Da        Isoelectric Point: 4.5714

>AT63836 WP_009615524.1 NZ_CP015878:750546-750950 [Pseudomonas citronellolis]
MKFPVVLHKDADSDYGVTIPDVPGCFSAGGTVSQALENVQEALALHFEGLVADGETLPQAQEVDTHMGNPDYAGGVWAVV
DFDVTPYLGKAVRFNASLPENLLQRIDERVKRDHRYASRSGFLATAALRELSSN

Download         Length: 405 bp

>AT63836 NZ_CP015878:750546-750950 [Pseudomonas citronellolis]
ATGAAATTCCCCGTGGTTCTGCACAAGGACGCCGACTCGGATTACGGCGTGACCATCCCTGACGTGCCTGGCTGCTTCTC
CGCCGGCGGCACCGTCAGCCAGGCGCTGGAAAACGTCCAGGAAGCCCTGGCCCTACACTTCGAAGGCCTGGTGGCCGACG
GCGAGACGCTCCCGCAAGCCCAGGAAGTGGACACCCACATGGGCAACCCCGACTACGCCGGCGGCGTCTGGGCGGTGGTC
GACTTCGACGTCACCCCCTACCTGGGCAAGGCCGTGCGCTTCAACGCCAGCCTCCCGGAAAACCTCCTGCAACGCATCGA
CGAACGCGTGAAGCGCGACCATCGCTACGCCTCTCGCTCCGGCTTCCTGGCTACCGCCGCGCTGCGTGAGTTGTCTTCGA
ACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127MLP4

Antitoxin

Source ID Structure
AlphaFold DB A0A1A5CY34

References