Detailed information of TA system TA063824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 910752..911338
• Replicon: chromosome
• Accession: NZ_CP015876
• Organism: Pseudomonas putida SJTE-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A210_RS04110
• Protein ID: WP_012051236.1
• Coordinates: 910752..911045 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: I3UY28
• Locus tag: A210_RS04115
• Protein ID: WP_004576347.1
• Coordinates: 911042..911338 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A210_RS04085	906355..907209	+	855	WP_014755376.1	putative selenate ABC transporter substrate-binding protein	-
A210_RS04090	907206..908015	+	810	WP_014755377.1	phosphonate ABC transporter ATP-binding protein	-
A210_RS04095	908009..908836	+	828	WP_003248575.1	ABC transporter permease subunit	-
A210_RS04100	908833..909600	+	768	WP_017148105.1	phosphonate ABC transporter, permease protein PhnE	-
A210_RS04105	909637..910530	-	894	WP_012051235.1	hypothetical protein	-
A210_RS04110	910752..911045	+	294	WP_012051236.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A210_RS04115	911042..911338	+	297	WP_004576347.1	putative addiction module antidote protein	Antitoxin
A210_RS04120	911349..911828	-	480	WP_012051237.1	GNAT family N-acetyltransferase	-
A210_RS04125	911999..912646	-	648	WP_017148106.1	serine protease	-
A210_RS04130	912989..913411	+	423	WP_012051239.1	VOC family protein	-
A210_RS04135	913996..915387	+	1392	WP_017148108.1	FAD-binding oxidoreductase	-
A210_RS04140	915467..915961	+	495	WP_014755382.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-92)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10648.42 Da
• Isoelectric Point: 10.6919

>T63824 WP_012051236.1 NZ_CP015876:910752-911045 [Pseudomonas putida SJTE-1]
MFLVEQTESFAAWLSSLRDLSARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVDFTQKGQRLVLLLVGGDKS
TQAADILKARKLLKEMK

Nucleotide

• Length: 294 bp

>T63824 NZ_CP015876:910752-911045 [Pseudomonas putida SJTE-1]
ATGTTTCTCGTTGAACAGACGGAAAGCTTTGCCGCTTGGCTATCGAGCTTGAGAGATTTGAGTGCCAGGCTGGCGATAGG
TCGTAGGCTGGAGCGAGCAGCGGCGGGCAATCTTGGTGACTTCAAGTGGCTTGGAGGCGGTATTGGTGAGCTTCGCATCG
ACGTAGCTGCCGGTTATCGCGTCGATTTCACGCAAAAGGGGCAGAGGTTGGTTTTACTGCTGGTCGGCGGCGACAAGTCC
ACCCAGGCCGCCGACATCCTCAAAGCCAGAAAGCTGCTGAAGGAGATGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10676.33 Da
• Isoelectric Point: 6.7249

>AT63824 WP_004576347.1 NZ_CP015876:911042-911338 [Pseudomonas putida SJTE-1]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT63824 NZ_CP015876:911042-911338 [Pseudomonas putida SJTE-1]
ATGAGCGAAAAATTGATCCCGTTTGATATGGCAGCCTTGTTGAGCAGCGATGAGGCAATCAGTGAATACCTTAGCCAGGT
GCTATCAAATGGCGATACCGAGGAAATCATTCGAGCACTTGGCCATATCGCTCGTGCACGGGGCATGACACATATTGCTA
CGGAGAGTGGGCTGGGTAGAGAAAGCCTGTACAAGGCGCTGAGCCCTGGCGCGAAGCCTCGTTTTGACACTGTATTGAGG
GTCATTCGTGCTCTGGGGGTGGACCTTCTCGTTCAGCCACATGCAATGACCCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CD26