Detailed information of TA system TA063823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 263811..264679
• Replicon: chromosome
• Accession: NZ_CP015876
• Organism: Pseudomonas putida SJTE-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A210_RS01145
• Protein ID: WP_172402800.1
• Coordinates: 263811..264164 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88R60
• Locus tag: A210_RS01150
• Protein ID: WP_004575922.1
• Coordinates: 264206..264679 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A210_RS01130	260115..260834	-	720	WP_010951640.1	response regulator	-
A210_RS01135	260975..263272	+	2298	WP_014755047.1	TonB-dependent siderophore receptor	-
A210_RS01140	263423..263629	-	207	WP_011953227.1	DUF3079 domain-containing protein	-
A210_RS01145	263811..264164	+	354	WP_172402800.1	type II toxin-antitoxin system HigB family toxin	Toxin
A210_RS01150	264206..264679	+	474	WP_004575922.1	transcriptional regulator	Antitoxin
A210_RS01155	265100..265315	+	216	WP_011953229.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A210_RS01160	265312..265635	-	324	WP_011953230.1	helix-turn-helix domain-containing protein	-
A210_RS01165	266160..267302	-	1143	WP_014755049.1	DUF4935 domain-containing protein	-
A210_RS01170	267406..268200	-	795	Protein_230	integrase	-
A210_RS01175	268198..269061	+	864	Protein_231	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13672.88 Da
• Isoelectric Point: 10.6182

>T63823 WP_172402800.1 NZ_CP015876:263811-264164 [Pseudomonas putida SJTE-1]
MRVITKAAVTKAIEAHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWVTVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 354 bp

>T63823 NZ_CP015876:263811-264164 [Pseudomonas putida SJTE-1]
ATGCGGGTCATAACGAAAGCGGCGGTAACCAAGGCGATTGAAGCCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCT
GACGACCTTCGACAGGGCGACGCTGCGGTTTGAATCTTTTGAACAGTTGAGGCAGACGTGGGTCACTGTGTCTGGCTGGA
ATGTTGATCGCATCCCCCACTCAAAACTTAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAG
AAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGCACTCTCTACATCAAGGCCATTCTGTCGCACGCCGA
GTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17229.67 Da
• Isoelectric Point: 4.4982

>AT63823 WP_004575922.1 NZ_CP015876:264206-264679 [Pseudomonas putida SJTE-1]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT63823 NZ_CP015876:264206-264679 [Pseudomonas putida SJTE-1]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CATTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGACAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CBJ4