Detailed information of TA system TA063819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 26451..27010
• Replicon: plasmid unnamed
• Accession: NZ_CP015854
• Organism: Escherichia coli strain ATCC 43889

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: A8714_RS28610
• Protein ID: WP_000421248.1
• Coordinates: 26735..27010 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: A8714_RS28605
• Protein ID: WP_001178089.1
• Coordinates: 26451..26735 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8714_RS28550	21643..22203	+	561	WP_000840472.1	fertility inhibition protein FinO	-
A8714_RS28555	22336..22548	+	213	WP_001302189.1	ANR family transcriptional regulator	-
A8714_RS28560	22793..23254	+	462	WP_001233856.1	thermonuclease family protein	-
A8714_RS28565	23300..23509	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
A8714_RS28570	23547..23885	+	339	WP_000766796.1	hypothetical protein	-
A8714_RS28575	24125..24379	+	255	WP_000083831.1	replication regulatory protein RepA	-
A8714_RS28585	24615..24689	+	75	WP_001370046.1	RepA leader peptide Tap	-
A8714_RS28590	24682..25539	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
A8714_RS28605	26451..26735	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
A8714_RS28610	26735..27010	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8714_RS28615	27105..27311	+	207	WP_001105064.1	hypothetical protein	-
A8714_RS28620	27411..27626	-	216	Protein_29	transposase	-
A8714_RS28625	27670..28236	-	567	Protein_30	IS3-like element IS600 family transposase	-
A8714_RS28630	28236..28652	-	417	Protein_31	IS91 family transposase	-
A8714_RS28635	28652..28837	-	186	WP_001302179.1	hypothetical protein	-
A8714_RS28640	29014..31224	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
A8714_RS28645	31268..31657	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / hlyD	1..92854	92854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T63819 WP_000421248.1 NZ_CP015854:26735-27010 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T63819 NZ_CP015854:26735-27010 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT63819 WP_001178089.1 NZ_CP015854:26451-26735 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT63819 NZ_CP015854:26451-26735 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8