Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4404500..4405154 Replicon chromosome
Accession NZ_CP015853
Organism Escherichia coli strain ATCC 43889

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag A8714_RS22440 Protein ID WP_000244781.1
Coordinates 4404747..4405154 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A8714_RS22435 Protein ID WP_000354046.1
Coordinates 4404500..4404766 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8714_RS22415 4400588..4402021 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
A8714_RS22420 4402066..4402377 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
A8714_RS22425 4402541..4403200 + 660 WP_000250274.1 hemolysin III family protein -
A8714_RS22430 4403277..4404257 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
A8714_RS22435 4404500..4404766 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A8714_RS22440 4404747..4405154 + 408 WP_000244781.1 protein YgfX Toxin
A8714_RS22445 4405194..4405715 - 522 WP_001055874.1 flavodoxin FldB -
A8714_RS22450 4405827..4406723 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A8714_RS22455 4406748..4407458 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A8714_RS22460 4407464..4409197 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T63817 WP_000244781.1 NZ_CP015853:4404747-4405154 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T63817 NZ_CP015853:4404747-4405154 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT63817 WP_000354046.1 NZ_CP015853:4404500-4404766 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT63817 NZ_CP015853:4404500-4404766 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References