Detailed information of TA system TA063778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 688865..689461
• Replicon: chromosome
• Accession: NZ_CP015852
• Organism: Pseudomonas koreensis strain CRS05-R5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A8L59_RS03015
• Protein ID: WP_064585767.1
• Coordinates: 689159..689461 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W6VRS3
• Locus tag: A8L59_RS03010
• Protein ID: WP_003221244.1
• Coordinates: 688865..689155 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L59_RS02990	685167..687287	-	2121	WP_064585763.1	TonB-dependent copper receptor	-
A8L59_RS02995	687373..687819	-	447	WP_064585764.1	DUF2946 domain-containing protein	-
A8L59_RS03000	687841..688320	-	480	WP_064585765.1	copper chaperone PCu(A)C	-
A8L59_RS03005	688369..688767	-	399	WP_064585766.1	DUF2946 domain-containing protein	-
A8L59_RS03010	688865..689155	-	291	WP_003221244.1	putative addiction module antidote protein	Antitoxin
A8L59_RS03015	689159..689461	-	303	WP_064585767.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8L59_RS03020	689621..690349	-	729	WP_064585768.1	cobalt-precorrin-6A reductase	-
A8L59_RS03025	690346..691443	-	1098	WP_064585769.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
A8L59_RS03030	691436..692647	-	1212	WP_064585770.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
A8L59_RS03035	692690..694078	+	1389	WP_082920275.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11582.24 Da
• Isoelectric Point: 8.0411

>T63778 WP_064585767.1 NZ_CP015852:c689461-689159 [Pseudomonas koreensis]
MIELERSRIFSEWLDSLKDIIGKGRIISRLRAAEHGNFGDCGFVGDAVYEMRVHYGPGYRMYFTRRDEVIYLLLIGGDKS
TQRRDIKRAVQMAHSIGNEE

Nucleotide

• Length: 303 bp

>T63778 NZ_CP015852:c689461-689159 [Pseudomonas koreensis]
ATGATCGAACTCGAACGATCCCGCATATTTTCCGAATGGCTCGACTCCTTGAAAGACATCATCGGCAAAGGCCGAATCAT
CTCCAGACTTCGAGCAGCCGAACATGGCAACTTTGGCGATTGCGGATTTGTCGGTGACGCCGTCTATGAAATGCGTGTTC
ACTACGGTCCTGGTTACAGGATGTACTTCACTCGTCGAGACGAAGTGATTTATCTGCTCTTGATCGGCGGCGACAAATCA
ACACAAAGGCGTGACATCAAACGCGCCGTACAAATGGCACACAGCATCGGTAATGAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10430.91 Da
• Isoelectric Point: 4.6027

>AT63778 WP_003221244.1 NZ_CP015852:c689155-688865 [Pseudomonas koreensis]
MTEKFTRFDAAEYLKTPEDMAAYLDACFDDDEGDGVLIRAALNDIARAQGMTQVARDAGLGRESLYKALSSTGNPEFATI
MKVMKALGLRLHAVAV

Nucleotide

• Length: 291 bp

>AT63778 NZ_CP015852:c689155-688865 [Pseudomonas koreensis]
ATGACCGAAAAATTCACCCGTTTCGACGCCGCCGAGTACCTTAAAACCCCGGAGGACATGGCGGCTTATCTGGACGCTTG
CTTTGACGATGATGAAGGCGACGGCGTCCTCATTCGTGCAGCACTCAATGACATAGCCCGAGCCCAGGGCATGACCCAAG
TGGCACGTGATGCGGGTCTGGGCCGTGAAAGTCTCTATAAAGCATTGAGCAGCACTGGCAATCCTGAATTCGCAACTATC
ATGAAAGTCATGAAGGCCTTGGGTTTGAGATTGCACGCTGTTGCGGTTTGA

Structures

Antitoxin

• AlphaFold DB: W6VRS3