Detailed information of TA system TA063777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 285886..286529
• Replicon: chromosome
• Accession: NZ_CP015852
• Organism: Pseudomonas koreensis strain CRS05-R5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1A9J8M0
• Locus tag: A8L59_RS01235
• Protein ID: WP_064585473.1
• Coordinates: 285886..286068 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A8L59_RS01240
• Protein ID: WP_169844064.1
• Coordinates: 286065..286529 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L59_RS01220	281789..282715	+	927	WP_064585470.1	serine acetyltransferase	-
A8L59_RS01225	283238..285199	+	1962	WP_169842759.1	choline transporter BetT	-
A8L59_RS01230	285223..285483	-	261	WP_064585472.1	hypothetical protein	-
A8L59_RS01235	285886..286068	+	183	WP_064585473.1	type II toxin-antitoxin system HicA family toxin	Toxin
A8L59_RS01240	286065..286529	+	465	WP_169844064.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A8L59_RS01245	286630..287667	-	1038	WP_064585474.1	LacI family DNA-binding transcriptional regulator	-
A8L59_RS01250	287873..290098	+	2226	WP_064585475.1	TonB-dependent receptor	-
A8L59_RS01255	290100..291419	+	1320	WP_064585476.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (24-148)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6858.98 Da
• Isoelectric Point: 10.3203

>T63777 WP_064585473.1 NZ_CP015852:285886-286068 [Pseudomonas koreensis]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHPCKSGRVTVKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T63777 NZ_CP015852:285886-286068 [Pseudomonas koreensis]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGCTGGTATCTGGTTGCAGTGAAGGGCAGTCACCATCAATA
CAAGCATCCGTGCAAGTCGGGTCGGGTGACGGTCAAGCATCCGGATTCGGATCTTCCCAAAGGGACGATCAACAGCATTT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16880.89 Da
• Isoelectric Point: 4.4234

>AT63777 WP_169844064.1 NZ_CP015852:286065-286529 [Pseudomonas koreensis]
MRPVIGNESPFMRTSQEGFTSMKFPVVLHKDADSDYGVIVPDVPGCFSAGTTVAEAFENTQEALALHYEGLVADGAPLPR
VQDIDAHLDNPDYAGGIWGVVDFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 465 bp

>AT63777 NZ_CP015852:286065-286529 [Pseudomonas koreensis]
ATGAGGCCCGTGATTGGAAATGAAAGTCCTTTTATGAGGACGTCACAGGAAGGATTCACGTCTATGAAATTCCCGGTCGT
TCTGCACAAGGATGCCGATTCGGACTACGGAGTGATTGTCCCGGATGTACCGGGGTGCTTCTCCGCAGGTACTACGGTAG
CCGAAGCGTTTGAAAACACTCAGGAGGCCCTGGCCTTGCACTATGAGGGACTGGTGGCAGATGGTGCACCGTTGCCTCGA
GTTCAGGACATTGATGCTCATCTCGACAATCCCGATTACGCGGGTGGAATCTGGGGCGTGGTGGACTTTGATATCACGCC
CTATTTCGGCAAATCGGTACGTTTCAACGCCACGCTGCCGGAACAACTGCTTGAGCGTATCGATCAGACAGTCCGGCGTG
ATCAGCGCTACAGCTCCCGTTCCGGTTTTCTGGCCGCAGCTGCGTTACGCGAGTTGTCGGCATAA

Structures

Toxin

• AlphaFold DB: A0A1A9J8M0