Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 462144..462777 Replicon chromosome
Accession NZ_CP015850
Organism Ralstonia solanacearum strain YC40-M

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag A3768_RS02150 Protein ID WP_016725172.1
Coordinates 462364..462777 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag A3768_RS02145 Protein ID WP_011002949.1
Coordinates 462144..462377 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3768_RS02130 457732..459027 - 1296 WP_011002952.1 MFS transporter -
A3768_RS02135 459035..460285 - 1251 WP_096761512.1 AGE family epimerase/isomerase -
A3768_RS02140 460480..462087 + 1608 WP_020832850.1 glucan biosynthesis protein -
A3768_RS02145 462144..462377 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A3768_RS02150 462364..462777 + 414 WP_016725172.1 PIN domain-containing protein Toxin
A3768_RS02155 462752..463378 - 627 WP_016725173.1 DUF1415 domain-containing protein -
A3768_RS02160 463569..463940 + 372 WP_016725174.1 hypothetical protein -
A3768_RS02165 464005..465501 - 1497 WP_016725175.1 glycerol kinase GlpK -
A3768_RS02170 465663..467399 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T63774 WP_016725172.1 NZ_CP015850:462364-462777 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T63774 NZ_CP015850:462364-462777 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT63774 WP_011002949.1 NZ_CP015850:462144-462377 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT63774 NZ_CP015850:462144-462377 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References