Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 53060..53619 | Replicon | plasmid p0157 |
| Accession | NZ_CP015847 | ||
| Organism | Escherichia coli O157:H7 strain FRIK2069 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | A8V30_RS30720 | Protein ID | WP_000421248.1 |
| Coordinates | 53060..53335 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | A8V30_RS30725 | Protein ID | WP_001178089.1 |
| Coordinates | 53335..53619 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A8V30_RS30680 | 48413..48802 | - | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
| A8V30_RS30685 | 48846..51056 | - | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| A8V30_RS30690 | 51233..51418 | + | 186 | WP_001302179.1 | hypothetical protein | - |
| A8V30_RS30695 | 51418..51834 | + | 417 | Protein_47 | IS91 family transposase | - |
| A8V30_RS30700 | 51834..52400 | + | 567 | Protein_48 | IS3-like element IS600 family transposase | - |
| A8V30_RS30705 | 52444..52659 | + | 216 | Protein_49 | transposase | - |
| A8V30_RS30715 | 52759..52965 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| A8V30_RS30720 | 53060..53335 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| A8V30_RS30725 | 53335..53619 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| A8V30_RS30740 | 54531..55388 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| A8V30_RS30745 | 55381..55455 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| A8V30_RS30755 | 55691..55945 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| A8V30_RS30760 | 56185..56523 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| A8V30_RS30765 | 56561..56770 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| A8V30_RS30770 | 56816..57277 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| A8V30_RS30775 | 57522..57734 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| A8V30_RS30780 | 57867..58427 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB | 1..95599 | 95599 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T63772 WP_000421248.1 NZ_CP015847:c53335-53060 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T63772 NZ_CP015847:c53335-53060 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT63772 WP_001178089.1 NZ_CP015847:c53619-53335 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT63772 NZ_CP015847:c53619-53335 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |