Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3575..4100 Replicon plasmid p0157
Accession NZ_CP015847
Organism Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A8V30_RS30435 Protein ID WP_001159868.1
Coordinates 3575..3880 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A8V30_RS30440 Protein ID WP_000813634.1
Coordinates 3882..4100 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8V30_RS30410 1..756 - 756 WP_000852148.1 replication initiation protein RepE -
A8V30_RS30420 832..2045 + 1214 WP_162829202.1 IS3 family transposase -
A8V30_RS30430 2768..3574 - 807 WP_000016989.1 site-specific integrase -
A8V30_RS30435 3575..3880 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A8V30_RS30440 3882..4100 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A8V30_RS30450 4559..5242 + 684 WP_010891291.1 DNA-binding protein -
A8V30_RS30455 5239..5859 + 621 WP_001248529.1 hypothetical protein -
A8V30_RS30460 5856..6056 + 201 WP_000708307.1 hypothetical protein -
A8V30_RS30470 6464..7441 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A8V30_RS30475 7726..8466 - 741 WP_064261959.1 tyrosine-type recombinase/integrase -
A8V30_RS30480 8587..8817 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB 1..95599 95599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T63771 WP_001159868.1 NZ_CP015847:c3880-3575 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T63771 NZ_CP015847:c3880-3575 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT63771 WP_000813634.1 NZ_CP015847:c4100-3882 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT63771 NZ_CP015847:c4100-3882 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References