Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4521621..4522252 Replicon chromosome
Accession NZ_CP015846
Organism Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag A8V30_RS24800 Protein ID WP_001259385.1
Coordinates 4521977..4522252 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A8V30_RS24795 Protein ID WP_000593555.1
Coordinates 4521621..4521980 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8V30_RS24765 4516733..4517206 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
A8V30_RS24770 4517203..4517484 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
A8V30_RS24775 4517561..4518919 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
A8V30_RS24780 4518912..4520420 + 1509 WP_001301773.1 carbohydrate kinase -
A8V30_RS24785 4520410..4520679 + 270 WP_000933060.1 HPr family phosphocarrier protein -
A8V30_RS24790 4520711..4521571 + 861 WP_001131788.1 class II aldolase -
A8V30_RS24795 4521621..4521980 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A8V30_RS24800 4521977..4522252 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
A8V30_RS24805 4522325..4523449 - 1125 WP_001301659.1 ABC transporter permease -
A8V30_RS24810 4523449..4526184 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
A8V30_RS24815 4526181..4527248 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T63762 WP_001259385.1 NZ_CP015846:c4522252-4521977 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T63762 NZ_CP015846:c4522252-4521977 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT63762 WP_000593555.1 NZ_CP015846:c4521980-4521621 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT63762 NZ_CP015846:c4521980-4521621 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References