Detailed information of TA system TA063746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2246318..2246789
• Replicon: chromosome
• Accession: NZ_CP015846
• Organism: Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: A8V30_RS12270
• Protein ID: WP_001303511.1
• Coordinates: 2246511..2246789 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: A8V30_RS12265
• Protein ID: WP_001302048.1
• Coordinates: 2246318..2246509 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V30_RS12225	2241525..2242109	-	585	WP_000206793.1	DUF551 domain-containing protein	-
A8V30_RS12230	2242166..2242561	-	396	WP_001118159.1	DUF977 family protein	-
A8V30_RS12235	2242577..2243346	-	770	Protein_2213	DUF1627 domain-containing protein	-
A8V30_RS12240	2243372..2244112	-	741	WP_000788936.1	ATP-binding protein	-
A8V30_RS12245	2244119..2245081	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
A8V30_RS12250	2245104..2245529	-	426	WP_000693943.1	Rha family transcriptional regulator	-
A8V30_RS12255	2245526..2245828	-	303	WP_000172738.1	transcriptional regulator	-
A8V30_RS12260	2245926..2246297	+	372	WP_001169686.1	hypothetical protein	-
A8V30_RS12265	2246318..2246509	+	192	WP_001302048.1	hypothetical protein	Antitoxin
A8V30_RS12270	2246511..2246789	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8V30_RS12280	2247142..2247441	+	300	WP_001240334.1	hypothetical protein	-
A8V30_RS12285	2247513..2247731	+	219	WP_001171930.1	hypothetical protein	-
A8V30_RS12295	2248300..2248488	+	189	WP_000449192.1	cell division inhibition protein DicB	-
A8V30_RS12300	2248485..2248676	+	192	WP_001090200.1	DUF1482 family protein	-
A8V30_RS12305	2248769..2251240	+	2472	WP_000048458.1	exonuclease	-
A8V30_RS12310	2251313..2251564	+	252	WP_000005552.1	excisionase family protein	-
A8V30_RS31315	2251599..2251783	+	185	Protein_2227	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2172411..2261669	89258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T63746 WP_001303511.1 NZ_CP015846:2246511-2246789 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T63746 NZ_CP015846:2246511-2246789 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT63746 WP_001302048.1 NZ_CP015846:2246318-2246509 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT63746 NZ_CP015846:2246318-2246509 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0