Detailed information of TA system TA063734

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1159744..1160539
• Replicon: chromosome
• Accession: NZ_CP015846
• Organism: Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: A8V30_RS05980
• Protein ID: WP_000854914.1
• Coordinates: 1160165..1160539 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LM11
• Locus tag: A8V30_RS05975
• Protein ID: WP_001280953.1
• Coordinates: 1159744..1160118 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V30_RS05930	1155099..1156004	+	906	WP_000203541.1	chemotaxis protein	-
A8V30_RS05935	1156001..1157071	+	1071	WP_000102669.1	patatin-like phospholipase family protein	-
A8V30_RS05950	1157411..1158229	+	819	WP_001234682.1	DUF945 domain-containing protein	-
A8V30_RS05955	1158320..1158805	+	486	WP_000214398.1	antirestriction protein	-
A8V30_RS05960	1158821..1159297	+	477	WP_001186738.1	RadC family protein	-
A8V30_RS05965	1159360..1159581	+	222	WP_000692323.1	DUF987 domain-containing protein	-
A8V30_RS05975	1159744..1160118	+	375	WP_001280953.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A8V30_RS05980	1160165..1160539	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
A8V30_RS05985	1160536..1161027	+	492	WP_000976857.1	hypothetical protein	-
A8V30_RS05990	1161039..1161236	+	198	WP_000839282.1	DUF957 domain-containing protein	-
A8V30_RS05995	1161321..1162163	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
A8V30_RS06005	1162654..1162872	-	219	WP_001040187.1	translation initiation factor IF-1	-
A8V30_RS06015	1163157..1163861	-	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T63734 WP_000854914.1 NZ_CP015846:1160165-1160539 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T63734 NZ_CP015846:1160165-1160539 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13734.56 Da
• Isoelectric Point: 6.6249

>AT63734 WP_001280953.1 NZ_CP015846:1159744-1160118 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Nucleotide

• Length: 375 bp

>AT63734 NZ_CP015846:1159744-1160118 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9LM11