Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | cptAB/- |
| Location | 3040416..3041070 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | cptA | Uniprot ID | Q46824 |
| Locus tag | b2896 | Protein ID | NP_417372.1 |
| Coordinates | 3040416..3040823 (-) | Length | 136 a.a. |
Antitoxin (Protein)
| Gene name | cptB | Uniprot ID | P64559 |
| Locus tag | b2897 | Protein ID | NP_417373.1 |
| Coordinates | 3040804..3041070 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b2892 | 3036373..3038106 | - | 1734 | NP_417368.1 | ssDNA-specific exonuclease RecJ | - |
| b2893 | 3038112..3038822 | - | 711 | NP_417369.1 | protein disulfide isomerase DsbC | - |
| b2894 | 3038847..3039743 | - | 897 | NP_417370.1 | site-specific recombinase | - |
| b2895 | 3039855..3040376 | + | 522 | NP_417371.1 | flavodoxin 2 | - |
| b2896 | 3040416..3040823 | - | 408 | NP_417372.1 | protein YgfX | Toxin |
| b2897 | 3040804..3041070 | - | 267 | NP_417373.1 | FAD assembly factor | Antitoxin |
| b2898 | 3041313..3042293 | + | 981 | NP_417374.1 | folate-binding protein | - |
| b2899 | 3042489..3043148 | - | 660 | NP_417375.1 | hemolysin-III family protein YqfA | - |
| b2900 | 3043312..3043623 | - | 312 | NP_417376.1 | N(4)-acetylcytidine amidohydrolase | - |
| b2901 | 3043662..3045101 | + | 1440 | NP_417377.1 | 6-phospho-beta-glucosidase A | - |
| b2902 | 3045158..3045901 | - | 744 | NP_417378.1 | putative oxidoreductase YgfF | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 136 a.a. Molecular weight: 16031.96 Da Isoelectric Point: 11.5202
>T6368 NP_417372.1 NC_000913:c3040823-3040416 [Escherichia coli str. K-12 substr. MG1655]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR
Download Length: 408 bp
>T6368 NC_000913:c3040823-3040416 [Escherichia coli str. K-12 substr. MG1655]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10547.12 Da Isoelectric Point: 5.1599
>AT6368 NP_417373.1 NC_000913:c3041070-3040804 [Escherichia coli str. K-12 substr. MG1655]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI
Download Length: 267 bp
>AT6368 NC_000913:c3041070-3040804 [Escherichia coli str. K-12 substr. MG1655]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10045 | Shewanella oneidensis MR-1 |
53.086 |
98.78 |
0.524 |
Multiple sequence alignment
References
(1) Matthew B McNeil et al. (2013) YgfX (CptA) is a multimeric membrane protein that interacts with the succinate dehydrogenase assembly factor SdhE (YgfY). Microbiology (Reading, England) 159(Pt 7):1352-1365. [PubMed:23657679]
(2) Hisako Masuda et al. (2012) A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia coli. FEMS Microbiology Letters 328(2):174-81. [PubMed:22239607]