Detailed information of TA system TA063660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2246311..2246782
• Replicon: chromosome
• Accession: NZ_CP015842
• Organism: Escherichia coli O157:H7 strain FRIK2533

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: A8V31_RS12235
• Protein ID: WP_001303511.1
• Coordinates: 2246504..2246782 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: A8V31_RS12230
• Protein ID: WP_001302048.1
• Coordinates: 2246311..2246502 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V31_RS12190	2241518..2242102	-	585	WP_000206793.1	DUF551 domain-containing protein	-
A8V31_RS12195	2242159..2242554	-	396	WP_001118159.1	DUF977 family protein	-
A8V31_RS12200	2242570..2243339	-	770	Protein_2214	DUF1627 domain-containing protein	-
A8V31_RS12205	2243365..2244105	-	741	WP_000788936.1	ATP-binding protein	-
A8V31_RS12210	2244112..2245074	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
A8V31_RS12215	2245097..2245522	-	426	WP_000693943.1	Rha family transcriptional regulator	-
A8V31_RS12220	2245519..2245821	-	303	WP_000172738.1	transcriptional regulator	-
A8V31_RS12225	2245919..2246290	+	372	WP_001169686.1	hypothetical protein	-
A8V31_RS12230	2246311..2246502	+	192	WP_001302048.1	hypothetical protein	Antitoxin
A8V31_RS12235	2246504..2246782	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8V31_RS12245	2247135..2247434	+	300	WP_001240334.1	hypothetical protein	-
A8V31_RS12250	2247506..2247724	+	219	WP_001171930.1	hypothetical protein	-
A8V31_RS12260	2248293..2248481	+	189	WP_000449192.1	cell division inhibition protein DicB	-
A8V31_RS12265	2248478..2248669	+	192	WP_001090200.1	DUF1482 family protein	-
A8V31_RS12270	2248762..2251233	+	2472	WP_000048458.1	exonuclease	-
A8V31_RS12275	2251306..2251557	+	252	WP_000005552.1	excisionase family protein	-
A8V31_RS30910	2251592..2251776	+	185	Protein_2228	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2176335..2261662	85327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T63660 WP_001303511.1 NZ_CP015842:2246504-2246782 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T63660 NZ_CP015842:2246504-2246782 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT63660 WP_001302048.1 NZ_CP015842:2246311-2246502 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT63660 NZ_CP015842:2246311-2246502 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0