Detailed information of TA system TA063638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 56385..56986
• Replicon: plasmid pMS6198C
• Accession: NZ_CP015837
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9Z1Q0
• Locus tag: MS6198_RS27540
• Protein ID: WP_001216047.1
• Coordinates: 56606..56986 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MS6198_RS27535
• Protein ID: WP_001190712.1
• Coordinates: 56385..56606 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS27480	51438..51626	+	189	WP_000797276.1	hypothetical protein	-
MS6198_RS27485	51628..51837	+	210	WP_000951710.1	hypothetical protein	-
MS6198_RS27490	51834..53111	+	1278	WP_068880404.1	DUF551 domain-containing protein	-
MS6198_RS27495	53194..53427	+	234	WP_000516537.1	hypothetical protein	-
MS6198_RS27500	53606..53899	+	294	WP_000269004.1	hypothetical protein	-
MS6198_RS27505	53906..54280	+	375	WP_068880407.1	hypothetical protein	-
MS6198_RS27510	54262..54912	+	651	WP_000057451.1	hypothetical protein	-
MS6198_RS27515	54896..55180	+	285	WP_001142394.1	hypothetical protein	-
MS6198_RS29895	55165..55374	-	210	WP_001344848.1	hypothetical protein	-
MS6198_RS27525	55548..55799	+	252	WP_001283837.1	DNA polymerase III subunit theta	-
MS6198_RS27530	55923..56312	+	390	WP_000506726.1	DNA repair protein	-
MS6198_RS27535	56385..56606	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MS6198_RS27540	56606..56986	+	381	WP_001216047.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MS6198_RS27545	56991..57170	+	180	WP_000113019.1	hypothetical protein	-
MS6198_RS27550	57198..58241	+	1044	WP_000644102.1	DUF968 domain-containing protein	-
MS6198_RS27555	58330..58782	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
MS6198_RS27560	58869..60062	+	1194	WP_032335092.1	hypothetical protein	-
MS6198_RS27565	60062..61546	+	1485	WP_000124150.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98242	98242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T63638 WP_001216047.1 NZ_CP015837:56606-56986 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T63638 NZ_CP015837:56606-56986 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT63638 WP_001190712.1 NZ_CP015837:56385-56606 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT63638 NZ_CP015837:56385-56606 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: U9Z1Q0

Antitoxin

• AlphaFold DB: A0A829CJB6