Detailed information of TA system TA063632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 66207..67010
• Replicon: plasmid pMS6198B
• Accession: NZ_CP015836
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: MS6198_RS26765
• Protein ID: WP_000348883.1
• Coordinates: 66207..66737 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: MS6198_RS26770
• Protein ID: WP_001275013.1
• Coordinates: 66741..67010 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS26745	61694..62179	-	486	WP_001403958.1	hypothetical protein	-
MS6198_RS26750	62570..65191	+	2622	WP_001586230.1	hypothetical protein	-
MS6198_RS26755	65390..65620	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MS6198_RS26760	65709..65984	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
MS6198_RS31335	65989..66206	-	218	Protein_77	transposase	-
MS6198_RS26765	66207..66737	-	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
MS6198_RS26770	66741..67010	-	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
MS6198_RS26775	67898..68887	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
MS6198_RS26780	69210..69950	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
MS6198_RS31340	70071..70253	-	183	WP_042004909.1	hypothetical protein	-
MS6198_RS26790	70320..70481	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MS6198_RS26795	70597..70865	-	269	Protein_84	type II toxin-antitoxin system ParD family antitoxin	-
MS6198_RS26800	71161..71901	-	741	WP_029702141.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / blaTEM-1B / blaCTX-M-15 / erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..136327	136327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T63632 WP_000348883.1 NZ_CP015836:c66737-66207 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T63632 NZ_CP015836:c66737-66207 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT63632 WP_001275013.1 NZ_CP015836:c67010-66741 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT63632 NZ_CP015836:c67010-66741 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7