Detailed information of TA system TA063624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4938110..4938705
• Replicon: chromosome
• Accession: NZ_CP015834
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: MS6198_RS24295
• Protein ID: WP_000239581.1
• Coordinates: 4938355..4938705 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: MS6198_RS24290
• Protein ID: WP_001223213.1
• Coordinates: 4938110..4938361 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS24280	4933775..4937554	+	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-
MS6198_RS24285	4937557..4937898	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
MS6198_RS24290	4938110..4938361	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
MS6198_RS24295	4938355..4938705	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
MS6198_RS24300	4938784..4939314	-	531	WP_000055075.1	inorganic diphosphatase	-
MS6198_RS24305	4939624..4940580	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
MS6198_RS24310	4940713..4942215	+	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
MS6198_RS24315	4942229..4943251	+	1023	WP_001298067.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T63624 WP_000239581.1 NZ_CP015834:4938355-4938705 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T63624 NZ_CP015834:4938355-4938705 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT63624 WP_001223213.1 NZ_CP015834:4938110-4938361 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT63624 NZ_CP015834:4938110-4938361 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7