Detailed information of TA system TA063603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 919697..920402
• Replicon: chromosome
• Accession: NZ_CP015834
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MS6198_RS04510
• Protein ID: WP_000539521.1
• Coordinates: 920016..920402 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MS6198_RS04505
• Protein ID: WP_001280945.1
• Coordinates: 919697..920026 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS04490	914855..915766	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
MS6198_RS04495	915943..918291	+	2349	WP_022645413.1	EAL domain-containing protein	-
MS6198_RS04500	918299..919627	+	1329	WP_022645414.1	GGDEF domain-containing protein	-
MS6198_RS04505	919697..920026	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MS6198_RS04510	920016..920402	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS6198_RS04515	920628..921953	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MS6198_RS04520	922166..922549	+	384	WP_000497137.1	biofilm formation regulator BssR	-
MS6198_RS04525	922660..923775	+	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
MS6198_RS04530	923772..924398	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T63603 WP_000539521.1 NZ_CP015834:c920402-920016 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T63603 NZ_CP015834:c920402-920016 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT63603 WP_001280945.1 NZ_CP015834:c920026-919697 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT63603 NZ_CP015834:c920026-919697 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6