Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58616..59166 Replicon chromosome
Accession NZ_CP015834
Organism Escherichia coli strain MS6198

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MS6198_RS00285 Protein ID WP_001160966.1
Coordinates 58852..59166 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag MS6198_RS00280 Protein ID WP_000125568.1
Coordinates 58616..58849 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6198_RS00255 53690..53977 + 288 WP_000203741.1 ferredoxin-like protein FixX -
MS6198_RS00260 54035..55366 + 1332 WP_001183201.1 MFS transporter -
MS6198_RS00265 55474..56004 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MS6198_RS00270 55997..57859 + 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
MS6198_RS00275 58051..58530 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
MS6198_RS00280 58616..58849 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MS6198_RS00285 58852..59166 + 315 WP_001160966.1 CcdB family protein Toxin
MS6198_RS00290 59163..60011 - 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MS6198_RS00295 60018..60395 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MS6198_RS00300 60398..61219 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MS6198_RS00305 61216..62205 - 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MS6198_RS00310 62205..63491 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T63599 WP_001160966.1 NZ_CP015834:58852-59166 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T63599 NZ_CP015834:58852-59166 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT63599 WP_000125568.1 NZ_CP015834:58616-58849 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT63599 NZ_CP015834:58616-58849 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References