Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5049367..5049864 Replicon chromosome
Accession NZ_CP015832
Organism Escherichia coli O157 strain 180-PT54

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A8L39_RS32840 Protein ID WP_001173343.1
Coordinates 5049367..5049540 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A8L39_RS32845 Protein ID WP_000398619.1
Coordinates 5049568..5049864 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8L39_RS25885 5046037..5047494 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
A8L39_RS25890 5047731..5049248 + 1518 WP_001295074.1 lysine--tRNA ligase -
A8L39_RS32840 5049367..5049540 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A8L39_RS32845 5049568..5049864 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A8L39_RS25905 5050091..5050363 - 273 WP_000405647.1 N-acetyltransferase -
A8L39_RS25910 5050375..5050605 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
A8L39_RS25915 5050786..5052417 + 1632 WP_001216477.1 sensor histidine kinase -
A8L39_RS25920 5052414..5053133 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T63597 WP_001173343.1 NZ_CP015832:c5049540-5049367 [Escherichia coli O157]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T63597 NZ_CP015832:c5049540-5049367 [Escherichia coli O157]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT63597 WP_000398619.1 NZ_CP015832:c5049864-5049568 [Escherichia coli O157]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT63597 NZ_CP015832:c5049864-5049568 [Escherichia coli O157]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References