Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2867045..2867683 Replicon chromosome
Accession NZ_CP015832
Organism Escherichia coli O157 strain 180-PT54

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A8L39_RS15015 Protein ID WP_000813794.1
Coordinates 2867507..2867683 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A8L39_RS15010 Protein ID WP_001270286.1
Coordinates 2867045..2867461 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8L39_RS14990 2862197..2863138 - 942 WP_001251315.1 ABC transporter permease -
A8L39_RS14995 2863139..2864152 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
A8L39_RS15000 2864170..2865315 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
A8L39_RS15005 2865560..2866966 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
A8L39_RS15010 2867045..2867461 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A8L39_RS15015 2867507..2867683 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A8L39_RS15020 2867905..2868135 + 231 WP_000494244.1 YncJ family protein -
A8L39_RS15025 2868227..2870188 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A8L39_RS15030 2870261..2870797 - 537 WP_064234957.1 DNA-binding transcriptional regulator SutR -
A8L39_RS15035 2870850..2872061 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T63580 WP_000813794.1 NZ_CP015832:c2867683-2867507 [Escherichia coli O157]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T63580 NZ_CP015832:c2867683-2867507 [Escherichia coli O157]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT63580 WP_001270286.1 NZ_CP015832:c2867461-2867045 [Escherichia coli O157]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT63580 NZ_CP015832:c2867461-2867045 [Escherichia coli O157]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References