Detailed information of TA system TA063536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2629459..2629930
• Replicon: chromosome
• Accession: NZ_CP015831
• Organism: Escherichia coli O157 strain 644-PT8

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: A8L40_RS13650
• Protein ID: WP_001303511.1
• Coordinates: 2629459..2629737 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: A8L40_RS13655
• Protein ID: WP_001302048.1
• Coordinates: 2629739..2629930 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L40_RS34870	2624465..2624649	-	185	Protein_2595	DUF3596 domain-containing protein	-
A8L40_RS13615	2624684..2624935	-	252	WP_000005552.1	excisionase family protein	-
A8L40_RS13620	2625008..2627479	-	2472	WP_000048458.1	exonuclease	-
A8L40_RS13625	2627572..2627763	-	192	WP_001090200.1	DUF1482 family protein	-
A8L40_RS13630	2627760..2627948	-	189	WP_000449192.1	cell division inhibition protein DicB	-
A8L40_RS33150	2628349..2628513	+	165	WP_001331716.1	hypothetical protein	-
A8L40_RS13635	2628517..2628735	-	219	WP_001171930.1	hypothetical protein	-
A8L40_RS13640	2628807..2629106	-	300	WP_072143447.1	hypothetical protein	-
A8L40_RS13650	2629459..2629737	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8L40_RS13655	2629739..2629930	-	192	WP_001302048.1	hypothetical protein	Antitoxin
A8L40_RS33155	2629951..2630322	-	372	WP_001169686.1	hypothetical protein	-
A8L40_RS13665	2630420..2630722	+	303	WP_000172738.1	transcriptional regulator	-
A8L40_RS13670	2630719..2631144	+	426	WP_000693943.1	Rha family transcriptional regulator	-
A8L40_RS13675	2631167..2632129	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
A8L40_RS13680	2632136..2632876	+	741	WP_000788938.1	ATP-binding protein	-
A8L40_RS13690	2632902..2633671	+	770	Protein_2610	DUF1627 domain-containing protein	-
A8L40_RS13695	2633687..2634082	+	396	WP_001118159.1	DUF977 family protein	-
A8L40_RS13700	2634139..2634723	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T63536 WP_001303511.1 NZ_CP015831:c2629737-2629459 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T63536 NZ_CP015831:c2629737-2629459 [Escherichia coli O157]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT63536 WP_001302048.1 NZ_CP015831:c2629930-2629739 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT63536 NZ_CP015831:c2629930-2629739 [Escherichia coli O157]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0