Detailed information of TA system TA063518

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 135292..136093
• Replicon: chromosome
• Accession: NZ_CP015831
• Organism: Escherichia coli O157 strain 644-PT8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: A8L40_RS00740
• Protein ID: WP_001094437.1
• Coordinates: 135292..135669 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: A8L40_RS00745
• Protein ID: WP_001390338.1
• Coordinates: 135716..136093 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L40_RS33945	130439..130746	-	308	Protein_133	IS3 family transposase	-
A8L40_RS00710	131064..132602	-	1539	WP_000998051.1	IS66-like element ISEc8 family transposase	-
A8L40_RS00715	132652..132999	-	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
A8L40_RS00720	132996..133376	-	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
A8L40_RS00725	133660..134505	-	846	WP_001274561.1	DUF4942 domain-containing protein	-
A8L40_RS00730	134590..134787	-	198	WP_000772032.1	DUF957 domain-containing protein	-
A8L40_RS00735	134807..135295	-	489	WP_053920163.1	hypothetical protein	-
A8L40_RS00740	135292..135669	-	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
A8L40_RS00745	135716..136093	-	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A8L40_RS00755	136256..136477	-	222	WP_000692345.1	DUF987 domain-containing protein	-
A8L40_RS00760	136540..137016	-	477	WP_001535682.1	RadC family protein	-
A8L40_RS00765	137032..137505	-	474	WP_000855064.1	antirestriction protein	-
A8L40_RS00775	137847..138665	-	819	WP_001535681.1	DUF945 domain-containing protein	-
A8L40_RS00780	138820..138978	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T63518 WP_001094437.1 NZ_CP015831:c135669-135292 [Escherichia coli O157]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T63518 NZ_CP015831:c135669-135292 [Escherichia coli O157]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT63518 WP_001390338.1 NZ_CP015831:c136093-135716 [Escherichia coli O157]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT63518 NZ_CP015831:c136093-135716 [Escherichia coli O157]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1