Detailed information of TA system TA063517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 56209..56852
• Replicon: 2 plasmid 2
• Accession: NZ_CP015824
• Organism: Klebsiella pneumoniae isolate blood sample

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: A8N26_RS28965
• Protein ID: WP_001044770.1
• Coordinates: 56209..56625 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: A8N26_RS28970
• Protein ID: WP_001261282.1
• Coordinates: 56622..56852 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8N26_RS28935	52162..53346	-	1185	WP_004152398.1	ISAs1-like element ISKpn31 family transposase	-
A8N26_RS28940	53448..53678	+	231	Protein_54	IS5/IS1182 family transposase	-
A8N26_RS28945	53850..54209	+	360	WP_004152400.1	DUF305 domain-containing protein	-
A8N26_RS28950	54694..55391	-	698	Protein_56	IS1 family transposase	-
A8N26_RS28960	55425..56135	-	711	WP_004152334.1	ATP-binding protein	-
A8N26_RS28965	56209..56625	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8N26_RS28970	56622..56852	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A8N26_RS28975	56809..57270	+	462	WP_072093212.1	hypothetical protein	-
A8N26_RS28980	57505..57711	+	207	WP_004153649.1	hypothetical protein	-
A8N26_RS28985	57757..58065	+	309	WP_004152336.1	hypothetical protein	-
A8N26_RS28990	58093..58422	+	330	WP_004152337.1	hypothetical protein	-
A8N26_RS28995	58448..58846	+	399	WP_004171440.1	hypothetical protein	-
A8N26_RS29000	58853..59185	+	333	WP_004152339.1	hypothetical protein	-
A8N26_RS29005	59185..59967	+	783	WP_004152340.1	site-specific integrase	-
A8N26_RS30965	60859..61089	-	231	WP_011977773.1	hypothetical protein	-
A8N26_RS30970	61181..61654	-	474	WP_004152341.1	YkgJ family cysteine cluster protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-3	-	1..103147	103147
	-	flank	IS/Tn	-	-	61774..63042	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T63517 WP_001044770.1 NZ_CP015824:c56625-56209 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T63517 NZ_CP015824:c56625-56209 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT63517 WP_001261282.1 NZ_CP015824:c56852-56622 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT63517 NZ_CP015824:c56852-56622 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3