Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 44338..45074 Replicon 2 plasmid 1
Accession NZ_CP015823
Organism Klebsiella pneumoniae isolate blood sample

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag A8N26_RS27865 Protein ID WP_003026803.1
Coordinates 44592..45074 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag A8N26_RS27860 Protein ID WP_003026799.1
Coordinates 44338..44604 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8N26_RS27815 40400..40762 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
A8N26_RS27820 40812..41162 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
A8N26_RS27825 41520..41789 + 270 WP_004152102.1 hypothetical protein -
A8N26_RS27830 41777..42352 + 576 WP_004152103.1 hypothetical protein -
A8N26_RS27835 42383..42877 + 495 WP_004152104.1 DNA-binding protein -
A8N26_RS27840 42921..43289 + 369 WP_004152105.1 hypothetical protein -
A8N26_RS27845 43323..43526 + 204 WP_004152106.1 hemolysin expression modulator Hha -
A8N26_RS27850 43575..43832 + 258 WP_004152107.1 hypothetical protein -
A8N26_RS27855 43908..44162 + 255 WP_004152108.1 hypothetical protein -
A8N26_RS27860 44338..44604 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
A8N26_RS27865 44592..45074 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
A8N26_RS27870 45286..46632 + 1347 WP_020314316.1 ISNCY family transposase -
A8N26_RS31915 47133..48387 - 1255 Protein_50 IS3 family transposase -
A8N26_RS27885 48475..49437 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..205221 205221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T63516 WP_003026803.1 NZ_CP015823:44592-45074 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T63516 NZ_CP015823:44592-45074 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT63516 WP_003026799.1 NZ_CP015823:44338-44604 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT63516 NZ_CP015823:44338-44604 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References