Detailed information of TA system TA063516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 44338..45074
• Replicon: 2 plasmid 1
• Accession: NZ_CP015823
• Organism: Klebsiella pneumoniae isolate blood sample

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: A8N26_RS27865
• Protein ID: WP_003026803.1
• Coordinates: 44592..45074 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: A8N26_RS27860
• Protein ID: WP_003026799.1
• Coordinates: 44338..44604 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8N26_RS27815	40400..40762	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
A8N26_RS27820	40812..41162	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
A8N26_RS27825	41520..41789	+	270	WP_004152102.1	hypothetical protein	-
A8N26_RS27830	41777..42352	+	576	WP_004152103.1	hypothetical protein	-
A8N26_RS27835	42383..42877	+	495	WP_004152104.1	DNA-binding protein	-
A8N26_RS27840	42921..43289	+	369	WP_004152105.1	hypothetical protein	-
A8N26_RS27845	43323..43526	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
A8N26_RS27850	43575..43832	+	258	WP_004152107.1	hypothetical protein	-
A8N26_RS27855	43908..44162	+	255	WP_004152108.1	hypothetical protein	-
A8N26_RS27860	44338..44604	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
A8N26_RS27865	44592..45074	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
A8N26_RS27870	45286..46632	+	1347	WP_020314316.1	ISNCY family transposase	-
A8N26_RS31915	47133..48387	-	1255	Protein_50	IS3 family transposase	-
A8N26_RS27885	48475..49437	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1..205221	205221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T63516 WP_003026803.1 NZ_CP015823:44592-45074 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T63516 NZ_CP015823:44592-45074 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT63516 WP_003026799.1 NZ_CP015823:44338-44604 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT63516 NZ_CP015823:44338-44604 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66