Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4834193..4834812 Replicon chromosome
Accession NZ_CP015822
Organism Klebsiella pneumoniae isolate blood sample

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag A8N26_RS24455 Protein ID WP_002892050.1
Coordinates 4834193..4834411 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag A8N26_RS24460 Protein ID WP_002892066.1
Coordinates 4834438..4834812 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8N26_RS24420 4830240..4830503 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
A8N26_RS24425 4830503..4830643 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
A8N26_RS24430 4830640..4831338 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
A8N26_RS24435 4831439..4832890 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
A8N26_RS24440 4832865..4833335 - 471 WP_002892026.1 YlaC family protein -
A8N26_RS32560 4833356..4833496 + 141 WP_004147370.1 hypothetical protein -
A8N26_RS24450 4833468..4834034 - 567 WP_002892030.1 maltose O-acetyltransferase -
A8N26_RS24455 4834193..4834411 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
A8N26_RS24460 4834438..4834812 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
A8N26_RS24465 4835298..4838444 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
A8N26_RS24470 4838467..4839660 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T63515 WP_002892050.1 NZ_CP015822:c4834411-4834193 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T63515 NZ_CP015822:c4834411-4834193 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT63515 WP_002892066.1 NZ_CP015822:c4834812-4834438 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT63515 NZ_CP015822:c4834812-4834438 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References