Detailed information of TA system TA063488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2132256..2132785
• Replicon: chromosome
• Accession: NZ_CP015817
• Organism: Staphylococcus aureus strain FORC_039

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FORC39_RS11045
• Protein ID: WP_000621175.1
• Coordinates: 2132256..2132618 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FORC39_RS11050
• Protein ID: WP_000948331.1
• Coordinates: 2132615..2132785 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC39_RS11020	2129234..2130004	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
FORC39_RS11025	2129979..2130458	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FORC39_RS11030	2130460..2130786	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FORC39_RS11035	2130905..2131906	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FORC39_RS11045	2132256..2132618	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FORC39_RS11050	2132615..2132785	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FORC39_RS11055	2132870..2134018	-	1149	WP_001281154.1	alanine racemase	-
FORC39_RS11060	2134084..2134443	-	360	WP_000581197.1	holo-ACP synthase	-
FORC39_RS11065	2134447..2134938	-	492	WP_001205912.1	PH domain-containing protein	-
FORC39_RS11070	2134925..2136508	-	1584	WP_001294620.1	PH domain-containing protein	-
FORC39_RS11075	2136501..2136980	-	480	WP_001287079.1	hypothetical protein	-
FORC39_RS11080	2137189..2137749	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T63488 WP_000621175.1 NZ_CP015817:c2132618-2132256 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T63488 NZ_CP015817:c2132618-2132256 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT63488 WP_000948331.1 NZ_CP015817:c2132785-2132615 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT63488 NZ_CP015817:c2132785-2132615 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1