63476

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 964068..964747 Replicon chromosome
Accession NZ_CP015776
Organism Brucella pseudogrignonensis strain K8

Toxin (Protein)


Gene name vapC Uniprot ID A0A1A9FUC3
Locus tag A8A54_RS23120 Protein ID WP_064323174.1
Coordinates 964068..964493 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1A9FU95
Locus tag A8A54_RS23125 Protein ID WP_007880986.1
Coordinates 964490..964747 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8A54_RS23100 959377..960678 + 1302 WP_064323285.1 FAD-binding oxidoreductase -
A8A54_RS23105 960714..961193 + 480 WP_064323171.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
A8A54_RS23110 961195..962361 + 1167 WP_064323172.1 amidohydrolase -
A8A54_RS24220 962449..962712 + 264 Protein_921 transposase -
A8A54_RS23955 963532..963842 + 311 Protein_922 recombinase family protein -
A8A54_RS23120 964068..964493 - 426 WP_064323174.1 type II toxin-antitoxin system VapC family toxin Toxin
A8A54_RS23125 964490..964747 - 258 WP_007880986.1 hypothetical protein Antitoxin
A8A54_RS23130 964874..965884 - 1011 WP_064323175.1 IS30 family transposase -
A8A54_RS23135 966141..966437 - 297 WP_083898673.1 putative addiction module antidote protein -
A8A54_RS23140 966485..967009 + 525 WP_167659801.1 hypothetical protein -
A8A54_RS23145 967568..968512 + 945 WP_064323286.1 sugar-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-127)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15405.62 Da        Isoelectric Point: 5.5742

>T63476 WP_064323174.1 NZ_CP015776:c964493-964068 [Brucella pseudogrignonensis]
MIVIDTNVISEMWKVEPHPDALTWFDAQAIETLYLSAITVAELRYGLAAMPEGKRRTIYQQRLEHEVLPAFAGRVLAFDL
ATTKHYADLMAQARTSGKAIANADGYIAATAAAHGFIVATRDTSPFRAAGLEVIDPWATSV

Download         Length: 426 bp

>T63476 NZ_CP015776:c964493-964068 [Brucella pseudogrignonensis]
ATGATTGTCATCGACACCAACGTGATTTCCGAGATGTGGAAAGTCGAGCCACACCCCGACGCGCTGACGTGGTTCGATGC
ACAGGCCATTGAAACGCTTTACCTATCTGCCATCACTGTCGCCGAGTTGCGCTATGGCCTTGCGGCGATGCCAGAGGGGA
AGCGCCGCACGATTTATCAGCAACGACTGGAGCACGAGGTTTTGCCGGCCTTCGCTGGCCGCGTCCTGGCCTTCGACCTG
GCCACGACCAAGCACTACGCCGATCTGATGGCACAAGCGCGGACGTCTGGTAAGGCTATCGCCAACGCTGACGGCTACAT
CGCGGCTACTGCTGCGGCCCATGGCTTTATTGTGGCGACCCGCGATACCAGCCCGTTTCGAGCCGCGGGACTGGAGGTCA
TCGATCCCTGGGCGACTTCTGTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9393.64 Da        Isoelectric Point: 7.4064

>AT63476 WP_007880986.1 NZ_CP015776:c964747-964490 [Brucella pseudogrignonensis]
MANVTVRNLPDAVHRALRVRAAHHGRSTEAEIREILETAARPPQRVKLGSLLASIAREAGGLTDEEHAVFDSVRDKTPAE
PMRFE

Download         Length: 258 bp

>AT63476 NZ_CP015776:c964747-964490 [Brucella pseudogrignonensis]
ATGGCGAATGTGACCGTTAGAAATCTACCCGATGCTGTGCATCGGGCGCTTCGTGTACGCGCCGCCCACCACGGCCGTAG
CACCGAGGCGGAAATCCGCGAAATTCTCGAAACCGCCGCTCGACCGCCTCAGCGCGTCAAGCTGGGTTCCTTGCTGGCGT
CCATCGCCCGCGAGGCCGGCGGCCTGACGGATGAAGAGCACGCCGTATTTGACAGTGTGCGCGACAAGACGCCGGCCGAA
CCGATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9FUC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9FU95

References