Detailed information of TA system TA063475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 937439..937986
• Replicon: chromosome
• Accession: NZ_CP015776
• Organism: Brucella pseudogrignonensis strain K8

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: A8A54_RS22985
• Protein ID: WP_064323164.1
• Coordinates: 937741..937986 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: A8A54_RS22980
• Protein ID: WP_007881039.1
• Coordinates: 937439..937741 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8A54_RS22965	932797..933666	+	870	WP_084480004.1	fructoselysine 6-kinase	-
A8A54_RS22970	933669..935072	+	1404	WP_064323162.1	FAD-binding oxidoreductase	-
A8A54_RS22975	935116..936222	+	1107	WP_064323163.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
A8A54_RS24345	936898..937056	+	159	WP_007881041.1	hypothetical protein	-
A8A54_RS24410	937177..937350	+	174	WP_200860689.1	hypothetical protein	-
A8A54_RS22980	937439..937741	-	303	WP_007881039.1	BrnA antitoxin family protein	Antitoxin
A8A54_RS22985	937741..937986	-	246	WP_064323164.1	BrnT family toxin	Toxin
A8A54_RS22990	938062..938457	+	396	WP_007881037.1	hypothetical protein	-
A8A54_RS22995	939362..939622	+	261	WP_007881036.1	type II toxin-antitoxin system ParD family antitoxin	-
A8A54_RS23930	939600..939914	+	315	WP_208856215.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A8A54_RS23005	940369..941085	-	717	WP_007881034.1	anti-sigma factor	-
A8A54_RS23010	941073..941618	-	546	WP_007881033.1	sigma-70 family RNA polymerase sigma factor	-
A8A54_RS23015	941808..942602	+	795	WP_105520309.1	DUF4394 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (25-98)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8956.53 Da
• Isoelectric Point: 10.1834

>T63475 WP_064323164.1 NZ_CP015776:c937986-937741 [Brucella pseudogrignonensis]
MKIVWDEPKRAANLLKHGMDFASLTIEFFETSTVIPAKNGRLQAIGRLADGTIVVIFVTLGTEALSVISMRPARKDERSL
I

Nucleotide

• Length: 246 bp

>T63475 NZ_CP015776:c937986-937741 [Brucella pseudogrignonensis]
ATGAAGATCGTATGGGACGAACCAAAACGCGCGGCCAATCTTTTGAAGCACGGTATGGATTTTGCTTCGCTAACTATCGA
GTTTTTCGAAACTTCAACTGTCATTCCCGCCAAAAATGGCAGGCTTCAAGCTATTGGTCGCCTCGCAGACGGAACAATCG
TTGTTATCTTCGTGACCCTCGGCACAGAAGCGCTCTCCGTTATCTCAATGCGCCCTGCCCGAAAAGATGAAAGGAGCCTA
ATCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11424.09 Da
• Isoelectric Point: 10.3878

>AT63475 WP_007881039.1 NZ_CP015776:c937741-937439 [Brucella pseudogrignonensis]
MAINFNPKHAAEHGYIKEDWDTVSDNPPITKEEMKKAKPFKEALPAHYESIQRSRGRPKVPNPKEAVTLRLDPDVVEKFK
ATGKNWRRSMNEALRKAAGL

Nucleotide

• Length: 303 bp

>AT63475 NZ_CP015776:c937741-937439 [Brucella pseudogrignonensis]
ATGGCTATCAATTTTAATCCGAAACATGCTGCAGAGCATGGCTACATCAAAGAGGATTGGGATACGGTTTCAGACAATCC
TCCTATTACCAAAGAGGAAATGAAAAAGGCCAAACCATTCAAGGAAGCGCTACCAGCGCATTATGAAAGCATACAGCGCT
CACGCGGTCGGCCTAAGGTTCCCAACCCAAAAGAGGCGGTTACGCTGCGTCTTGATCCCGATGTTGTCGAAAAATTCAAG
GCAACGGGAAAAAACTGGCGCCGGAGTATGAACGAAGCACTACGCAAAGCCGCAGGCCTTTAA