Detailed information of TA system TA063474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 661443..662017
• Replicon: chromosome
• Accession: NZ_CP015776
• Organism: Brucella pseudogrignonensis strain K8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8A54_RS21640
• Protein ID: WP_064322980.1
• Coordinates: 661443..661820 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A8A54_RS21645
• Protein ID: WP_007881510.1
• Coordinates: 661817..662017 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8A54_RS21625	657066..658061	+	996	WP_083898702.1	iron chelate uptake ABC transporter family permease subunit	-
A8A54_RS21630	658058..659098	+	1041	WP_064322979.1	iron chelate uptake ABC transporter family permease subunit	-
A8A54_RS21635	659265..661004	-	1740	WP_007881512.1	gamma-glutamyltransferase	-
A8A54_RS21640	661443..661820	-	378	WP_064322980.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8A54_RS21645	661817..662017	-	201	WP_007881510.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
A8A54_RS21655	662579..664423	-	1845	WP_064323264.1	cadmium-translocating P-type ATPase	-
A8A54_RS21660	664533..665228	-	696	Protein_629	VIT family protein	-
A8A54_RS21665	665455..666753	+	1299	WP_064322982.1	TIGR01244 family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14115.33 Da
• Isoelectric Point: 6.3273

>T63474 WP_064322980.1 NZ_CP015776:c661820-661443 [Brucella pseudogrignonensis]
VILADTSIWIDHFRSGDNELIKIIADDLLLCHPAIIGELALGSLRDRTSVLAFLNVQREAEVATHDEVMTMIEKHCIFSM
GIGYTDAQLLASVLLDRRTSLWTRDKRLKVTAEKAGARLYERLHN

Nucleotide

• Length: 378 bp

>T63474 NZ_CP015776:c661820-661443 [Brucella pseudogrignonensis]
GTGATTTTGGCAGACACTTCAATTTGGATTGACCATTTCCGCAGCGGCGATAATGAGCTTATTAAGATTATCGCTGATGA
CCTTTTGCTTTGCCATCCAGCGATTATAGGCGAACTTGCGTTGGGCAGTCTCCGAGACAGGACGAGTGTATTGGCTTTTT
TGAATGTGCAGCGTGAAGCTGAAGTCGCAACACATGACGAAGTCATGACCATGATTGAAAAGCACTGCATTTTTAGCATG
GGCATTGGCTATACTGATGCCCAACTGCTGGCATCAGTTCTTCTCGACCGGCGAACTTCACTGTGGACCCGAGACAAGCG
CTTAAAGGTTACGGCTGAAAAAGCCGGTGCACGGCTTTACGAGCGGCTGCACAATTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7318.57 Da
• Isoelectric Point: 11.0199

>AT63474 WP_007881510.1 NZ_CP015776:c662017-661817 [Brucella pseudogrignonensis]
MRSTINLDDKLLEKARLLTGTKETAALVRQALETLVRLETGKRLIALGGTMPDVEAAPRRRSSEKK

Nucleotide

• Length: 201 bp

>AT63474 NZ_CP015776:c662017-661817 [Brucella pseudogrignonensis]
ATGCGCTCAACCATCAATCTTGATGACAAGCTTTTAGAAAAAGCCAGATTGTTGACCGGAACGAAAGAGACAGCCGCTTT
AGTCCGACAAGCGCTAGAAACACTTGTGCGACTAGAGACTGGTAAACGCCTTATTGCTCTTGGAGGCACGATGCCGGACG
TTGAAGCAGCTCCTCGCCGTCGGAGCTCAGAGAAGAAGTGA