Detailed information of TA system TA063473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 644744..645342
• Replicon: chromosome
• Accession: NZ_CP015776
• Organism: Brucella pseudogrignonensis strain K8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A8A54_RS23880
• Protein ID: WP_084479989.1
• Coordinates: 645049..645342 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1A9FTZ9
• Locus tag: A8A54_RS21565
• Protein ID: WP_064322969.1
• Coordinates: 644744..645052 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8A54_RS21550	641015..641860	+	846	WP_064322967.1	mechanosensitive ion channel	-
A8A54_RS21555	642158..643675	+	1518	WP_064322968.1	C69 family dipeptidase	-
A8A54_RS21560	644221..644498	-	278	Protein_610	transposase	-
A8A54_RS21565	644744..645052	+	309	WP_064322969.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
A8A54_RS23880	645049..645342	+	294	WP_084479989.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8A54_RS21570	645501..646574	-	1074	WP_064322970.1	hypothetical protein	-
A8A54_RS21580	647078..647326	+	249	WP_064322972.1	CopG family ribbon-helix-helix protein	-
A8A54_RS21585	647323..647625	+	303	WP_064322973.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A8A54_RS21590	648222..648917	+	696	WP_064322974.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-52)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10689.36 Da
• Isoelectric Point: 8.6391

>T63473 WP_084479989.1 NZ_CP015776:645049-645342 [Brucella pseudogrignonensis]
MKLTWSAFALSDRDVIFTFIEAENPSSAIMVDERIVAAARRLVDFPASGRVGRIAGTRELVINGTPYVVAYAVTQSAVRI
LRVLHGAREWPDMIPTS

Nucleotide

• Length: 294 bp

>T63473 NZ_CP015776:645049-645342 [Brucella pseudogrignonensis]
CTGAAGCTCACCTGGTCTGCATTTGCGTTATCAGATCGAGACGTCATCTTCACTTTCATTGAGGCGGAGAATCCCTCATC
TGCAATCATGGTCGACGAACGAATAGTCGCGGCTGCTCGTCGCCTAGTTGATTTTCCGGCGAGTGGTCGGGTTGGCAGAA
TTGCCGGGACGCGGGAGCTGGTGATAAACGGCACCCCATACGTCGTGGCTTATGCTGTAACACAATCGGCTGTTCGTATC
CTCCGCGTACTTCATGGCGCACGGGAATGGCCAGACATGATTCCTACCAGCTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11148.63 Da
• Isoelectric Point: 6.2296

>AT63473 WP_064322969.1 NZ_CP015776:644744-645052 [Brucella pseudogrignonensis]
MASNALVQTRIDAEVRDRASAVLESMGLTVSDVVRILLTRTANEGALPLELISGSEAHDAWFRSKVLEALHDTRPDVPDD
EVTAHFERRRAAARLKAAALKS

Nucleotide

• Length: 309 bp

>AT63473 NZ_CP015776:644744-645052 [Brucella pseudogrignonensis]
ATGGCTTCTAACGCACTCGTTCAAACTCGCATTGATGCAGAAGTTAGAGATCGTGCCTCTGCGGTTCTGGAAAGTATGGG
TCTTACGGTGTCGGACGTAGTTCGTATCTTACTCACACGGACGGCGAACGAGGGGGCACTACCGCTCGAACTTATATCAG
GCAGCGAAGCGCACGATGCTTGGTTTCGCAGCAAGGTTCTTGAAGCGCTTCACGACACGCGACCCGACGTGCCGGATGAT
GAAGTCACAGCTCATTTCGAAAGACGACGGGCGGCAGCGCGCCTTAAAGCAGCGGCGCTCAAGTCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1A9FTZ9