Detailed information of TA system TA063472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4986..5539
• Replicon: chromosome
• Accession: NZ_CP015776
• Organism: Brucella pseudogrignonensis strain K8

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: A8A54_RS18460
• Protein ID: WP_064323223.1
• Coordinates: 4986..5231 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: A8A54_RS18465
• Protein ID: WP_064322579.1
• Coordinates: 5231..5539 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8A54_RS18440	1359..2483	-	1125	WP_064322576.1	M20 family metallopeptidase	-
A8A54_RS18445	2702..3598	+	897	WP_064322577.1	LysR family transcriptional regulator	-
A8A54_RS18450	3830..4135	-	306	WP_064323222.1	hypothetical protein	-
A8A54_RS18460	4986..5231	+	246	WP_064323223.1	BrnT family toxin	Toxin
A8A54_RS18465	5231..5539	+	309	WP_064322579.1	BrnA antitoxin family protein	Antitoxin
A8A54_RS18470	5593..5886	+	294	WP_167659789.1	hypothetical protein	-
A8A54_RS18475	5896..6297	+	402	WP_064322581.1	hypothetical protein	-
A8A54_RS18480	6489..6809	-	321	WP_064322582.1	hypothetical protein	-
A8A54_RS18485	7029..8099	-	1071	WP_064322583.1	response regulator	-
A8A54_RS18490	8106..8672	-	567	WP_064322584.1	chemotaxis protein CheB	-
A8A54_RS18495	8669..9508	-	840	WP_064322585.1	chemotaxis protein CheR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-100)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8967.41 Da
• Isoelectric Point: 10.2728

>T63472 WP_064323223.1 NZ_CP015776:4986-5231 [Brucella pseudogrignonensis]
MNIVWDEPKRQSNLSKHGIDFADLSYEFFLAANVASTKQGRLKAIGRLADGTIVVIFITLGTEALSIISMRTANKVERSK
I

Nucleotide

• Length: 246 bp

>T63472 NZ_CP015776:4986-5231 [Brucella pseudogrignonensis]
ATGAACATCGTTTGGGACGAACCAAAAAGACAATCCAACTTATCCAAGCACGGGATTGATTTTGCAGACTTGTCTTATGA
ATTCTTTTTGGCTGCAAACGTAGCCAGCACAAAACAAGGGCGGCTAAAAGCTATTGGCCGTCTTGCTGATGGAACGATTG
TTGTCATCTTCATCACTCTCGGAACAGAAGCGCTTTCAATCATTTCCATGCGAACAGCAAATAAGGTCGAAAGGAGCAAA
ATCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11535.20 Da
• Isoelectric Point: 10.0084

>AT63472 WP_064322579.1 NZ_CP015776:5231-5539 [Brucella pseudogrignonensis]
VANKFRPKHAEAHGYTKEDWDAVDSPPLTDEELSSMKPFKEVFPEMAEKMEKAIAARGRPRLDTPKTPVTIRLDQDVVQR
FKASGKGWQGRMNEALRKAVGL

Nucleotide

• Length: 309 bp

>AT63472 NZ_CP015776:5231-5539 [Brucella pseudogrignonensis]
GTGGCTAACAAATTCAGACCTAAACATGCAGAAGCCCACGGCTATACCAAGGAGGATTGGGACGCAGTCGATAGCCCACC
GCTAACCGACGAAGAGCTATCCAGTATGAAGCCATTTAAGGAAGTTTTCCCCGAAATGGCGGAGAAAATGGAGAAGGCAA
TTGCAGCTCGCGGACGCCCTCGACTGGATACTCCTAAAACTCCGGTAACTATTCGTCTTGATCAAGATGTAGTCCAACGC
TTTAAGGCCAGCGGCAAAGGCTGGCAGGGCCGTATGAACGAAGCACTACGCAAAGCCGTGGGGCTTTAG