Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4579055..4579671 Replicon chromosome
Accession NZ_CP015774
Organism Lelliottia amnigena strain ZB04

Toxin (Protein)


Gene name vapC Uniprot ID A0A1A9FGI3
Locus tag A8A57_RS21885 Protein ID WP_064328014.1
Coordinates 4579055..4579426 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1A9FGH8
Locus tag A8A57_RS21890 Protein ID WP_064328015.1
Coordinates 4579429..4579671 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8A57_RS21870 4576555..4577457 + 903 WP_064328011.1 formate dehydrogenase subunit beta -
A8A57_RS21875 4577454..4578089 + 636 WP_064328012.1 formate dehydrogenase cytochrome b556 subunit -
A8A57_RS21880 4578086..4579015 + 930 WP_064328013.1 formate dehydrogenase accessory protein FdhE -
A8A57_RS21885 4579055..4579426 - 372 WP_064328014.1 type II toxin-antitoxin system VapC family toxin Toxin
A8A57_RS21890 4579429..4579671 - 243 WP_064328015.1 ribbon-helix-helix domain-containing protein Antitoxin
A8A57_RS21895 4579787..4580728 - 942 WP_064328016.1 fatty acid biosynthesis protein FabY -
A8A57_RS21900 4580773..4581210 - 438 WP_064328017.1 D-tyrosyl-tRNA(Tyr) deacylase -
A8A57_RS21905 4581207..4582079 - 873 WP_064328018.1 virulence factor BrkB family protein -
A8A57_RS21910 4582073..4582672 - 600 WP_064328019.1 glucose-1-phosphatase -
A8A57_RS21915 4582805..4583605 - 801 WP_064328020.1 DeoR/GlpR family DNA-binding transcription regulator -
A8A57_RS21920 4583640..4584536 - 897 WP_064328021.1 sugar kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13585.74 Da        Isoelectric Point: 6.6339

>T63471 WP_064328014.1 NZ_CP015774:c4579426-4579055 [Lelliottia amnigena]
MQHMAVFDTNILIDLFNNRHEAADALDNTASHCAISLITWMEVMVGARKYGQEAKTAAVMGAFEIIDVSREIAQRSVLLR
VQHGMKLPDAIILATAQIKHCALISRNTKDFAGIDGVFSPYQL

Download         Length: 372 bp

>T63471 NZ_CP015774:c4579426-4579055 [Lelliottia amnigena]
ATGCAGCACATGGCAGTGTTTGATACCAACATCCTGATCGATCTTTTCAATAATCGCCATGAGGCTGCGGACGCATTAGA
TAACACCGCTTCGCACTGTGCGATTAGCTTAATCACCTGGATGGAAGTGATGGTAGGCGCTCGCAAATATGGTCAAGAAG
CTAAAACAGCGGCGGTGATGGGAGCGTTTGAGATTATCGATGTGTCACGAGAGATCGCACAGAGGAGTGTTTTACTTCGC
GTTCAGCACGGTATGAAACTGCCGGATGCCATTATACTGGCCACTGCTCAAATCAAACATTGCGCGCTCATTTCACGCAA
TACCAAGGATTTCGCAGGCATTGACGGCGTGTTTTCGCCTTATCAACTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9576.86 Da        Isoelectric Point: 4.4076

>AT63471 WP_064328015.1 NZ_CP015774:c4579671-4579429 [Lelliottia amnigena]
MSLMAERDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQNSSVIRKALGLWSDQQEDGLEYERKMREEW

Download         Length: 243 bp

>AT63471 NZ_CP015774:c4579671-4579429 [Lelliottia amnigena]
ATGAGTCTCATGGCGGAACGTGATATGGGCAGAATACTGATTGATTTGTCTGACGATGTTATCCAGCGGCTGGATAACCT
CAAACAATTGCGCAATCAGCCCCGCGCTGAATTGCTGCGAGAAGCAATAGAGTTATACCTCGACCAACAAAACTCATCGG
TTATTCGCAAGGCTCTGGGTCTGTGGAGCGACCAGCAAGAAGATGGGCTTGAGTATGAACGCAAGATGCGAGAGGAGTGG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9FGI3

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9FGH8

References