Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2293403..2294216 Replicon chromosome
Accession NZ_CP015774
Organism Lelliottia amnigena strain ZB04

Toxin (Protein)


Gene name KacT Uniprot ID A0A1A9FC71
Locus tag A8A57_RS11020 Protein ID WP_064326120.1
Coordinates 2293403..2293942 (-) Length 180 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A1A9FBB7
Locus tag A8A57_RS11025 Protein ID WP_064326121.1
Coordinates 2293947..2294216 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8A57_RS10995 2288601..2289791 + 1191 WP_064326116.1 cytochrome c biogenesis protein/redoxin -
A8A57_RS11000 2289795..2290553 - 759 WP_064326117.1 trans-aconitate 2-methyltransferase -
A8A57_RS11005 2290696..2291496 + 801 WP_064326118.1 OBAP family protein -
A8A57_RS11010 2291620..2292627 - 1008 WP_064326119.1 hypothetical protein -
A8A57_RS11015 2292745..2293359 + 615 WP_071892373.1 TetR/AcrR family transcriptional regulator -
A8A57_RS11020 2293403..2293942 - 540 WP_064326120.1 GNAT family N-acetyltransferase Toxin
A8A57_RS11025 2293947..2294216 - 270 WP_064326121.1 DUF1778 domain-containing protein Antitoxin
A8A57_RS11030 2294606..2294830 - 225 Protein_2115 helix-turn-helix domain-containing protein -
A8A57_RS11035 2295091..2296614 + 1524 WP_064326122.1 site-specific integrase -
A8A57_RS11040 2296577..2298241 + 1665 WP_064326123.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 180 a.a.        Molecular weight: 20074.97 Da        Isoelectric Point: 8.8533

>T63461 WP_064326120.1 NZ_CP015774:c2293942-2293403 [Lelliottia amnigena]
VDVLKIAIFSEDVNYDLSNFDCGEAYLNTFLTDHLKRQHMGKVLRGYVLTTRENKPRVMGYYTLSGSCFEKEFLPSKTQQ
KRVPYKNVPSVTLGRLAVDKSLHRQGYGEALVTHAMKVVYHASQAVGIHGMFVEALNDNAKKFYLNLGFVLLRGANGNTL
FYPTTAIEELFDGKEANAG

Download         Length: 540 bp

>T63461 NZ_CP015774:c2293942-2293403 [Lelliottia amnigena]
GTGGACGTTTTAAAGATAGCGATTTTTTCTGAAGACGTTAATTATGATCTCAGCAACTTCGATTGCGGTGAAGCGTATCT
GAATACTTTTCTCACTGACCATCTGAAACGCCAGCATATGGGTAAGGTTTTACGTGGTTACGTGCTCACCACGAGAGAAA
ATAAACCCCGTGTTATGGGTTATTACACTTTGTCAGGAAGCTGCTTTGAAAAGGAGTTTCTGCCAAGTAAAACGCAACAA
AAACGCGTGCCCTACAAAAACGTTCCGAGTGTGACTCTGGGACGTCTGGCTGTTGATAAAAGCCTACATCGCCAGGGCTA
CGGAGAAGCGCTCGTGACGCATGCCATGAAGGTGGTGTATCACGCCTCTCAAGCGGTCGGCATTCATGGAATGTTCGTTG
AAGCGCTTAACGACAACGCCAAAAAATTCTATCTTAATCTGGGATTTGTACTGCTCAGGGGAGCAAACGGGAACACCCTC
TTCTATCCAACCACAGCGATAGAGGAATTATTCGACGGCAAAGAAGCTAACGCAGGATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10105.54 Da        Isoelectric Point: 8.8077

>AT63461 WP_064326121.1 NZ_CP015774:c2294216-2293947 [Lelliottia amnigena]
MSALKKQRIDLRLNEDDKSMIEEAAAMTNQSISQFMVSTASERAAEVINQHRRLILNEASWNLVMDAISNPPAPNDRLKR
AAKRLQNLE

Download         Length: 270 bp

>AT63461 NZ_CP015774:c2294216-2293947 [Lelliottia amnigena]
ATGTCAGCATTGAAGAAACAGCGCATCGATCTCAGATTAAACGAGGATGACAAAAGCATGATCGAAGAGGCTGCGGCAAT
GACTAACCAGTCAATTTCACAGTTTATGGTGAGCACCGCCTCTGAACGTGCAGCGGAAGTTATCAACCAGCATCGTCGAC
TGATCCTCAACGAGGCGTCGTGGAACCTCGTTATGGATGCAATCAGTAACCCACCGGCACCGAACGACCGACTGAAACGA
GCTGCTAAGCGTCTCCAGAATTTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9FC71

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9FBB7

References