Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1100354..1100973 Replicon chromosome
Accession NZ_CP015774
Organism Lelliottia amnigena strain ZB04

Toxin (Protein)


Gene name Hha Uniprot ID A0A1A9F9D9
Locus tag A8A57_RS05205 Protein ID WP_064325091.1
Coordinates 1100354..1100572 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1A9F8T6
Locus tag A8A57_RS05210 Protein ID WP_064325092.1
Coordinates 1100599..1100973 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8A57_RS05175 1096482..1096742 + 261 WP_064325086.1 type B 50S ribosomal protein L31 -
A8A57_RS05180 1096745..1096885 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
A8A57_RS05185 1096882..1097592 - 711 WP_064325087.1 GNAT family N-acetyltransferase -
A8A57_RS05190 1097693..1099153 + 1461 WP_064325088.1 PLP-dependent aminotransferase family protein -
A8A57_RS05195 1099125..1099592 - 468 WP_064325089.1 hypothetical protein -
A8A57_RS05200 1099710..1100261 - 552 WP_064325090.1 maltose O-acetyltransferase -
A8A57_RS05205 1100354..1100572 - 219 WP_064325091.1 hemolysin expression modulator Hha Toxin
A8A57_RS05210 1100599..1100973 - 375 WP_064325092.1 Hha toxicity modulator TomB Antitoxin
A8A57_RS05215 1101481..1104630 - 3150 WP_064325093.1 multidrug efflux RND transporter permease subunit AcrB -
A8A57_RS05220 1104653..1105849 - 1197 WP_064325094.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8646.02 Da        Isoelectric Point: 8.9008

>T63459 WP_064325091.1 NZ_CP015774:c1100572-1100354 [Lelliottia amnigena]
MSDKPLTKFDYLMRLRRCQSLDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T63459 NZ_CP015774:c1100572-1100354 [Lelliottia amnigena]
ATGTCAGATAAACCATTAACCAAATTTGATTATTTGATGCGTTTGCGACGTTGTCAGTCCCTCGATACGCTGGAACGCGT
GATTGAGAAAAATAAATACGAGCTTTCAGACAATGAACTGGCGGTCTTTTATTCGGCAGCCGATCATCGCCTTGCTGAAC
TGACGATGAATAAGCTCTACGATAAAATCCCAACCTCTGTGTGGAAATTCGTGCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14521.30 Da        Isoelectric Point: 4.8886

>AT63459 WP_064325092.1 NZ_CP015774:c1100973-1100599 [Lelliottia amnigena]
MDEYSPKRHDIAQLKFLCENLYHDCLANLNDSNHGWVNDPTSEINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSGYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT63459 NZ_CP015774:c1100973-1100599 [Lelliottia amnigena]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGTGAAAACCTGTACCATGACTGCCTTGC
CAACCTTAATGACAGCAATCATGGGTGGGTGAATGACCCAACCTCTGAAATCAATTTACAGCTCAATGAACTGATTGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATAGAGCAAATTGACGAATACCTG
GACGACACCTTTATGTTGTTCAGCGGTTACGGCATTAACGCACAAGATTTGCAAAAGTGGCGTAAATCGGGCAATCGACT
ATTTCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCTGTTAGTCTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9F9D9

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9F8T6

References