Detailed information of TA system TA063446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3031235..3031922
• Replicon: chromosome
• Accession: NZ_CP015773
• Organism: Mycobacterium tuberculosis variant bovis strain SP38

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: SZ58_RS14850
• Protein ID: WP_003414064.1
• Coordinates: 3031527..3031922 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: SZ58_RS14845
• Protein ID: WP_003414061.1
• Coordinates: 3031235..3031501 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SZ58_RS14820	3026874..3027776	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
SZ58_RS14825	3027845..3028597	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
SZ58_RS14830	3028841..3029116	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
SZ58_RS14835	3029113..3030735	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
SZ58_RS14840	3030822..3031238	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
SZ58_RS14845	3031235..3031501	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SZ58_RS14850	3031527..3031922	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
SZ58_RS14855	3031919..3032188	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
SZ58_RS14860	3032198..3033292	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
SZ58_RS14865	3033289..3033708	-	420	Protein_2801	winged helix-turn-helix domain-containing protein	-
SZ58_RS14870	3033782..3034261	-	480	WP_003414073.1	dihydrofolate reductase	-
SZ58_RS14875	3034332..3035132	-	801	WP_003911953.1	thymidylate synthase	-
SZ58_RS14880	3035288..3036025	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T63446 WP_003414064.1 NZ_CP015773:c3031922-3031527 [Mycobacterium tuberculosis variant bovis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T63446 NZ_CP015773:c3031922-3031527 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT63446 WP_003414061.1 NZ_CP015773:c3031501-3031235 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT63446 NZ_CP015773:c3031501-3031235 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64