Detailed information of TA system TA063434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2241356..2241982
• Replicon: chromosome
• Accession: NZ_CP015773
• Organism: Mycobacterium tuberculosis variant bovis strain SP38

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: SZ58_RS10830
• Protein ID: WP_003410075.1
• Coordinates: 2241584..2241982 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SZ58_RS10825
• Protein ID: WP_019283586.1
• Coordinates: 2241356..2241583 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SZ58_RS10815	2239395..2239739	-	345	WP_003410065.1	ferredoxin family protein	-
SZ58_RS10820	2239928..2241097	-	1170	WP_003899126.1	ATP-binding protein	-
SZ58_RS10825	2241356..2241583	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SZ58_RS10830	2241584..2241982	+	399	WP_003410075.1	PIN domain nuclease	Toxin
SZ58_RS10835	2242165..2242596	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
SZ58_RS10840	2242697..2243131	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
SZ58_RS10855	2243568..2243747	-	180	Protein_2050	hypothetical protein	-
SZ58_RS10860	2243808..2244455	+	648	WP_071854215.1	transposase	-
SZ58_RS10865	2244409..2244999	+	591	WP_003899131.1	IS110 family transposase	-
SZ58_RS10870	2245127..2246383	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T63434 WP_003410075.1 NZ_CP015773:2241584-2241982 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T63434 NZ_CP015773:2241584-2241982 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT63434 WP_019283586.1 NZ_CP015773:2241356-2241583 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT63434 NZ_CP015773:2241356-2241583 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG