Detailed information of TA system TA063428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2190673..2191376
• Replicon: chromosome
• Accession: NZ_CP015773
• Organism: Mycobacterium tuberculosis variant bovis strain SP38

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: SZ58_RS10505
• Protein ID: WP_003409778.1
• Coordinates: 2190673..2191002 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: SZ58_RS21285
• Protein ID: WP_003409780.1
• Coordinates: 2190999..2191376 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SZ58_RS10485	2187056..2188126	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
SZ58_RS10490	2188123..2188638	+	516	WP_003409718.1	flavin reductase family protein	-
SZ58_RS10495	2188635..2189696	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
SZ58_RS10500	2189693..2190463	+	771	WP_003409775.1	SDR family oxidoreductase	-
SZ58_RS10505	2190673..2191002	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SZ58_RS21285	2190999..2191376	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
SZ58_RS10515	2191373..2191963	-	591	WP_003409784.1	SEC-C domain-containing protein	-
SZ58_RS10520	2192018..2193382	+	1365	WP_031648120.1	HNH endonuclease	-
SZ58_RS10525	2193537..2193989	-	453	WP_010950636.1	lipoprotein	-
SZ58_RS10530	2194053..2194454	+	402	WP_003409869.1	hypothetical protein	-
SZ58_RS10535	2194447..2194629	-	183	WP_003409870.1	hypothetical protein	-
SZ58_RS10540	2194743..2195093	-	351	WP_003409871.1	hypothetical protein	-
SZ58_RS10545	2195104..2196006	-	903	WP_003409874.1	hypothetical protein	-
SZ58_RS10550	2196027..2196218	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T63428 WP_003409778.1 NZ_CP015773:c2191002-2190673 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T63428 NZ_CP015773:c2191002-2190673 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT63428 WP_003409780.1 NZ_CP015773:c2191376-2190999 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT63428 NZ_CP015773:c2191376-2190999 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8