Detailed information of TA system TA063415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015599..1016218
• Replicon: chromosome
• Accession: NZ_CP015773
• Organism: Mycobacterium tuberculosis variant bovis strain SP38

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: SZ58_RS04935
• Protein ID: WP_003404726.1
• Coordinates: 1015784..1016218 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: SZ58_RS04930
• Protein ID: WP_003404724.1
• Coordinates: 1015599..1015778 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SZ58_RS04920	1011072..1012652	+	1581	WP_011799139.1	serine hydrolase	-
SZ58_RS04925	1012649..1015042	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
SZ58_RS22080	1015315..1015473	+	159	WP_003404720.1	hypothetical protein	-
SZ58_RS04930	1015599..1015778	+	180	WP_003404724.1	antitoxin	Antitoxin
SZ58_RS04935	1015784..1016218	+	435	WP_003404726.1	SRPBCC family protein	Toxin
SZ58_RS04940	1016316..1017089	+	774	WP_003404735.1	VOC family protein	-
SZ58_RS04945	1017154..1017603	+	450	WP_003404738.1	hypothetical protein	-
SZ58_RS04950	1017738..1017968	-	231	WP_003898642.1	hypothetical protein	-
SZ58_RS04955	1018135..1019643	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
SZ58_RS04960	1019645..1020883	-	1239	WP_044292446.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T63415 WP_003404726.1 NZ_CP015773:1015784-1016218 [Mycobacterium tuberculosis variant bovis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T63415 NZ_CP015773:1015784-1016218 [Mycobacterium tuberculosis variant bovis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT63415 WP_003404724.1 NZ_CP015773:1015599-1015778 [Mycobacterium tuberculosis variant bovis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT63415 NZ_CP015773:1015599-1015778 [Mycobacterium tuberculosis variant bovis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5