Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 156935..157460 Replicon plasmid unnamed1
Accession NZ_CP015754
Organism Klebsiella pneumoniae strain W14

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag A7321_RS26395 Protein ID WP_004197633.1
Coordinates 156935..157240 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag A7321_RS26400 Protein ID WP_015632547.1
Coordinates 157242..157460 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7321_RS26380 152716..153999 + 1284 WP_064081523.1 DUF1173 family protein -
A7321_RS29090 154148..154453 + 306 WP_126034233.1 hypothetical protein -
A7321_RS26385 154775..155560 - 786 WP_048291886.1 site-specific integrase -
A7321_RS26390 155759..156748 - 990 WP_064081524.1 hypothetical protein -
A7321_RS26395 156935..157240 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
A7321_RS26400 157242..157460 - 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A7321_RS29095 157669..157875 - 207 Protein_147 hypothetical protein -
A7321_RS26405 158039..159127 + 1089 WP_032638120.1 hypothetical protein -
A7321_RS26410 159138..160511 + 1374 WP_063160997.1 hypothetical protein -
A7321_RS26415 160852..161280 - 429 WP_023345594.1 nuclear transport factor 2 family protein -
A7321_RS26420 161393..162238 - 846 WP_023345593.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..165275 165275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T63371 WP_004197633.1 NZ_CP015754:c157240-156935 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T63371 NZ_CP015754:c157240-156935 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCTCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT63371 WP_015632547.1 NZ_CP015754:c157460-157242 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT63371 NZ_CP015754:c157460-157242 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References