Detailed information of TA system TA063370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 134447..135186
• Replicon: plasmid unnamed1
• Accession: NZ_CP015754
• Organism: Klebsiella pneumoniae strain W14

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: A7321_RS26255
• Protein ID: WP_021312536.1
• Coordinates: 134447..134932 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: A7321_RS26260
• Protein ID: WP_003026799.1
• Coordinates: 134920..135186 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7321_RS26215	129492..129908	-	417	WP_074193590.1	relaxosome protein TraM	-
A7321_RS26220	130299..130784	+	486	WP_001568108.1	transglycosylase SLT domain-containing protein	-
A7321_RS26225	130817..131146	-	330	WP_074193592.1	hypothetical protein	-
A7321_RS26230	131179..132000	-	822	WP_064081511.1	DUF945 domain-containing protein	-
A7321_RS26240	132628..132978	-	351	WP_064081512.1	hypothetical protein	-
A7321_RS26245	132975..133247	-	273	WP_032445785.1	hypothetical protein	-
A7321_RS26250	133943..134104	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
A7321_RS26255	134447..134932	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
A7321_RS26260	134920..135186	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
A7321_RS28265	135319..135747	-	429	WP_004901287.1	GFA family protein	-
A7321_RS28580	135948..137316	-	1369	Protein_123	IS3 family transposase	-
A7321_RS26275	137522..137761	-	240	WP_064081514.1	hypothetical protein	-
A7321_RS26285	137985..138716	-	732	WP_064081515.1	plasmid SOS inhibition protein A	-
A7321_RS26290	138713..139144	-	432	WP_004194235.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..165275	165275
	-	flank	IS/Tn	-	-	135948..136580	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T63370 WP_021312536.1 NZ_CP015754:c134932-134447 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T63370 NZ_CP015754:c134932-134447 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT63370 WP_003026799.1 NZ_CP015754:c135186-134920 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT63370 NZ_CP015754:c135186-134920 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66