Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1469877..1470491 Replicon chromosome
Accession NZ_CP015750
Organism Pectobacterium wasabiae CFBP 3304

Toxin (Protein)


Gene name hicA Uniprot ID A0A1D7YZ62
Locus tag A7983_RS06610 Protein ID WP_005973346.1
Coordinates 1469877..1470056 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1D7YZ96
Locus tag A7983_RS06615 Protein ID WP_005973344.1
Coordinates 1470084..1470491 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7983_RS06585 1465120..1466280 - 1161 WP_005973357.1 cystathionine gamma-synthase -
A7983_RS06590 1466467..1466784 + 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
A7983_RS06595 1467003..1467800 + 798 WP_005973352.1 ABC transporter ATP-binding protein -
A7983_RS06600 1467810..1468772 + 963 WP_005973350.1 ABC transporter substrate-binding protein -
A7983_RS06605 1468769..1469770 + 1002 WP_005973348.1 iron ABC transporter permease -
A7983_RS06610 1469877..1470056 + 180 WP_005973346.1 type II toxin-antitoxin system HicA family toxin Toxin
A7983_RS06615 1470084..1470491 + 408 WP_005973344.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A7983_RS06620 1470684..1470899 - 216 WP_005973342.1 50S ribosomal protein L31 -
A7983_RS06625 1471212..1473410 + 2199 WP_005973340.1 primosomal protein N' -
A7983_RS06630 1473681..1474724 + 1044 WP_005973338.1 DNA-binding transcriptional regulator CytR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6530.61 Da        Isoelectric Point: 10.7838

>T63344 WP_005973346.1 NZ_CP015750:1469877-1470056 [Pectobacterium wasabiae CFBP 3304]
MDSRTLIAEIKADGWELIRVNGSHHHFTHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T63344 NZ_CP015750:1469877-1470056 [Pectobacterium wasabiae CFBP 3304]
ATGGATAGCAGGACGCTAATCGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTTAACGGAAGCCACCACCATTT
CACTCATCCCAGCAAACCGGGATTAGTCACCATTCCCCACCCTAAGAAGGATTTGCCGATAGGTACGGTAAAAAGCATCA
GGAAACAGGCAGGAATATGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14491.46 Da        Isoelectric Point: 4.3494

>AT63344 WP_005973344.1 NZ_CP015750:1470084-1470491 [Pectobacterium wasabiae CFBP 3304]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGETLDDAIANAKEAITGHIELLVEMGQDIPTVSTVGQLAKGAEYTGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERIYPK

Download         Length: 408 bp

>AT63344 NZ_CP015750:1470084-1470491 [Pectobacterium wasabiae CFBP 3304]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACTCACGCATACGGCGTAACAGTGCCAGATCTGCCAGGCTGTTT
TTCTGCGGGCGAGACGCTAGACGATGCCATCGCGAATGCTAAAGAAGCGATTACCGGGCATATCGAACTACTGGTCGAGA
TGGGTCAGGATATTCCCACCGTGTCAACAGTAGGGCAGTTGGCAAAAGGCGCAGAATACACTGGGTACACCTGGGCGGTT
GTTGATATCGATGTGACTCGCTTAATGGGCGGTTCAGAAAAAATTAACGTCACATTACCTAAGTCACTGATTGACCGAAT
TGATCGTTGCGTAGCCAGTAACCCAGAATTTAAAAGTCGTTCGGGTTTTCTCGCGCAGGCTGCATTAGAGCGTATATACC
CCAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7YZ62

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7YZ96

References