Detailed information of TA system TA063343

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 22582..23307
• Replicon: chromosome
• Accession: NZ_CP015750
• Organism: Pectobacterium wasabiae CFBP 3304

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A1D7YVT6
• Locus tag: A7983_RS00145
• Protein ID: WP_005974485.1
• Coordinates: 22582..22902 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A1D7YVT5
• Locus tag: A7983_RS00150
• Protein ID: WP_005974482.1
• Coordinates: 22972..23307 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7983_RS00120	18217..19228	+	1012	Protein_21	IS3 family transposase	-
A7983_RS00125	19240..20499	-	1260	Protein_22	integrase arm-type DNA-binding domain-containing protein	-
A7983_RS00130	20879..21307	-	429	WP_005974495.1	tRNA(fMet)-specific endonuclease VapC	-
A7983_RS00135	21307..21537	-	231	WP_005974492.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
A7983_RS00140	21631..22464	-	834	Protein_25	DUF4942 domain-containing protein	-
A7983_RS00145	22582..22902	-	321	WP_005974485.1	TA system toxin CbtA family protein	Toxin
A7983_RS00150	22972..23307	-	336	WP_005974482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A7983_RS00155	23347..23820	-	474	WP_005974480.1	DNA repair protein RadC	-
A7983_RS00160	23943..24293	-	351	WP_005974477.1	hypothetical protein	-
A7983_RS00165	24351..25184	-	834	WP_005974474.1	hypothetical protein	-
A7983_RS23795	25331..25489	-	159	WP_156105178.1	hypothetical protein	-
A7983_RS00170	25673..26560	-	888	WP_005974466.1	50S ribosome-binding GTPase	-
A7983_RS00175	26669..27289	-	621	WP_005974464.1	hypothetical protein	-
A7983_RS00180	27253..27678	-	426	WP_152413560.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11959.72 Da
• Isoelectric Point: 5.0187

>T63343 WP_005974485.1 NZ_CP015750:c22902-22582 [Pectobacterium wasabiae CFBP 3304]
MQTSPAIPPREDNPCPSPIAVWQQLLTYLLEKHYGLTLNDTPFCGENVIQAYIDAGITLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T63343 NZ_CP015750:c22902-22582 [Pectobacterium wasabiae CFBP 3304]
ATGCAAACATCACCGGCAATCCCTCCACGGGAGGATAACCCCTGTCCGTCACCTATAGCCGTCTGGCAGCAACTACTGAC
TTACCTGCTGGAAAAGCACTACGGCCTGACGCTGAACGACACACCATTCTGTGGGGAAAACGTGATTCAGGCGTATATCG
ATGCTGGCATCACGCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTTACCGCCGTTGATGTTCTCCGTGCCAGACGTGCTACTGGCTTGCTCAAGGCATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12462.17 Da
• Isoelectric Point: 5.7100

>AT63343 WP_005974482.1 NZ_CP015750:c23307-22972 [Pectobacterium wasabiae CFBP 3304]
MPSSETPEWGLKSAVTPRFGARLVQEGNRLHYLADRASIVGTFSKTETQSLERCFPELIKLLEQKLRMGELNPRQQNCVT
QHCNEWICEADTLGSVGYVYIVIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT63343 NZ_CP015750:c23307-22972 [Pectobacterium wasabiae CFBP 3304]
ATGCCATCATCGGAGACCCCGGAATGGGGACTAAAAAGTGCTGTCACGCCGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCACTATCTGGCAGACCGGGCCAGCATTGTCGGTACGTTTAGCAAAACAGAAACCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTAATTAAGCTGTTGGAACAGAAGCTACGCATGGGGGAACTGAATCCCCGTCAGCAGAACTGCGTCACG
CAGCATTGCAACGAATGGATCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGTTATCTATCCCTCTTC
GGCAGCAGCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A1D7YVT6

Antitoxin

• AlphaFold DB: A0A1D7YVT5