Detailed information of TA system TA063342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 20879..21537
• Replicon: chromosome
• Accession: NZ_CP015750
• Organism: Pectobacterium wasabiae CFBP 3304

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7983_RS00130
• Protein ID: WP_005974495.1
• Coordinates: 20879..21307 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1D7YVT7
• Locus tag: A7983_RS00135
• Protein ID: WP_005974492.1
• Coordinates: 21307..21537 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7983_RS00100	16790..16906	+	117	Protein_19	transposase	-
A7983_RS22925	17129..18151	-	1023	Protein_20	IS110 family transposase	-
A7983_RS00120	18217..19228	+	1012	Protein_21	IS3 family transposase	-
A7983_RS00125	19240..20499	-	1260	Protein_22	integrase arm-type DNA-binding domain-containing protein	-
A7983_RS00130	20879..21307	-	429	WP_005974495.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A7983_RS00135	21307..21537	-	231	WP_005974492.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A7983_RS00140	21631..22464	-	834	Protein_25	DUF4942 domain-containing protein	-
A7983_RS00145	22582..22902	-	321	WP_005974485.1	TA system toxin CbtA family protein	-
A7983_RS00150	22972..23307	-	336	WP_005974482.1	type IV toxin-antitoxin system YeeU family antitoxin	-
A7983_RS00155	23347..23820	-	474	WP_005974480.1	DNA repair protein RadC	-
A7983_RS00160	23943..24293	-	351	WP_005974477.1	hypothetical protein	-
A7983_RS00165	24351..25184	-	834	WP_005974474.1	hypothetical protein	-
A7983_RS23795	25331..25489	-	159	WP_156105178.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15874.09 Da
• Isoelectric Point: 7.2740

>T63342 WP_005974495.1 NZ_CP015750:c21307-20879 [Pectobacterium wasabiae CFBP 3304]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQNYGFDAAVHT
GQIRAELAKQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGRQADNSQSL

Nucleotide

• Length: 429 bp

>T63342 NZ_CP015750:c21307-20879 [Pectobacterium wasabiae CFBP 3304]
ATGCTGAAATATCTGCTTGATACCAATATCTGTATTTACACTATCAAGAACAAACCACAGGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGTTGATGGAATTGATCTATGGTGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTGGCCGTGATTGAAGGGTTTTCTGCGCGTCTGGAAGTTCAAAACTATGGTTTTGACGCCGCAGTGCATACC
GGCCAAATTCGTGCGGAACTGGCAAAACAAGGAACACCCATCGGCCCGTATGATGCCATGCTAGCGGGTCACGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAGTTTGAGCGTGTGCCAGGATTGCGCGTAGAAAACTGGGTCGGTA
GACAGGCTGACAATAGCCAGTCTTTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8666.74 Da
• Isoelectric Point: 4.4091

>AT63342 WP_005974492.1 NZ_CP015750:c21537-21307 [Pectobacterium wasabiae CFBP 3304]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEGESVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT63342 NZ_CP015750:c21537-21307 [Pectobacterium wasabiae CFBP 3304]
ATGGAAAAAACTACAGTATTTAAAAGCAATCGCAGTCAGGCGGTACGACTCCCTAAAGCGGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGCCGTACACGTATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTTGAAG
GGGAGAGCGTAACACCTGACTTCATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7YVT7