Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 3570950..3571986 Replicon chromosome
Accession NZ_CP015749
Organism Pectobacterium parmentieri strain RNS 08-42-1A

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A8B3FNB9
Locus tag A8F97_RS16170 Protein ID WP_033070784.1
Coordinates 3570950..3571525 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A8B3FHU5
Locus tag A8F97_RS16175 Protein ID WP_033070783.1
Coordinates 3571525..3571986 (-) Length 154 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8F97_RS16155 3566771..3568033 - 1263 WP_033070786.1 integrase arm-type DNA-binding domain-containing protein -
A8F97_RS23420 3568575..3568787 + 213 WP_082218588.1 DUF1133 family protein -
A8F97_RS16160 3568849..3569673 + 825 WP_127087856.1 hypothetical protein -
A8F97_RS16165 3569660..3570781 + 1122 WP_033070785.1 XRE family transcriptional regulator -
A8F97_RS16170 3570950..3571525 - 576 WP_033070784.1 PIN domain-containing protein Toxin
A8F97_RS16175 3571525..3571986 - 462 WP_033070783.1 helix-turn-helix domain-containing protein Antitoxin
A8F97_RS23425 3572291..3572509 + 219 Protein_3224 hypothetical protein -
A8F97_RS23435 3572678..3572752 - 75 Protein_3225 type II toxin-antitoxin system RelE/ParE family toxin -
A8F97_RS24195 3572967..3573218 + 252 WP_127087855.1 hypothetical protein -
A8F97_RS16185 3573765..3574643 + 879 WP_033070781.1 integrase domain-containing protein -
A8F97_RS16190 3574646..3574846 + 201 WP_014698606.1 AlpA family transcriptional regulator -
A8F97_RS23440 3574843..3575379 + 537 WP_033070780.1 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3564060..3581066 17006


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-115)

Antitoxin

SinI-like, DNA-binding domain

(84-131)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21771.27 Da        Isoelectric Point: 4.8050

>T63340 WP_033070784.1 NZ_CP015749:c3571525-3570950 [Pectobacterium parmentieri]
MNHSPYPVVLDACVIYPNMLRDLLMEVGKSGLYQPKWTATIHDEWQRNLVENVPDLTREKLKRVEDLMNKALPDALVTGF
ENLMEGVSLPDLDDRHVVAAAIRSDSEVIVTFNLKDFPKESLAEFDIEAIHPDEFIADLLDLNQALVLQAVQRQRARLKN
PKKSVDEYFDILLKLGLPMTVKAITAYKLMI

Download         Length: 576 bp

>T63340 NZ_CP015749:c3571525-3570950 [Pectobacterium parmentieri]
ATGAATCATTCGCCTTACCCAGTCGTACTTGATGCCTGTGTCATTTATCCCAACATGCTCCGCGATCTGCTTATGGAGGT
TGGGAAATCGGGTCTGTATCAACCTAAATGGACGGCCACCATTCATGATGAATGGCAAAGAAATTTGGTTGAAAACGTAC
CGGATTTAACGCGTGAAAAATTGAAGCGCGTAGAGGATCTGATGAACAAAGCCCTACCTGATGCTCTTGTAACGGGCTTT
GAAAATCTTATGGAAGGGGTTTCACTCCCAGATCTTGACGATCGCCATGTTGTAGCTGCAGCGATAAGATCCGATTCAGA
GGTGATCGTAACCTTCAATCTTAAAGACTTTCCAAAGGAGAGCCTGGCTGAGTTTGATATTGAAGCAATTCATCCTGATG
AGTTTATCGCTGATTTGCTGGATTTAAATCAAGCCTTAGTTTTACAGGCTGTCCAAAGGCAGCGAGCCCGGCTAAAGAAC
CCCAAAAAATCAGTAGATGAATATTTCGACATACTACTGAAACTAGGCTTGCCGATGACGGTTAAGGCTATAACTGCTTA
TAAATTAATGATTTAG

Antitoxin


Download         Length: 154 a.a.        Molecular weight: 17321.80 Da        Isoelectric Point: 6.1440

>AT63340 WP_033070783.1 NZ_CP015749:c3571986-3571525 [Pectobacterium parmentieri]
MTSSILDNVSLPAQKEIEVALRGQRELATFLTTKFETQKISIHDANDEKHQIELPTSALRLLMNILGELAEGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEEGKLPHHKTGRHRRVLFADLMRYKAQRETESNEAMQELVDLSQELGFY

Download         Length: 462 bp

>AT63340 NZ_CP015749:c3571986-3571525 [Pectobacterium parmentieri]
ATGACTAGCTCAATTCTGGACAATGTAAGCCTTCCGGCTCAGAAGGAAATTGAGGTTGCGTTACGTGGACAAAGAGAACT
GGCAACATTTCTCACCACAAAGTTCGAAACGCAAAAAATATCGATTCACGATGCAAACGACGAAAAACACCAGATCGAAT
TGCCAACCTCAGCACTGAGATTGCTAATGAACATCTTGGGTGAACTCGCTGAAGGGAACGCGGTTCAAGTTGTCCCTGTG
CACGCTGAATTAACTACACAGGAGGCCGCCAATATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTTTAGAAGAAGG
AAAGCTTCCACATCATAAAACAGGCCGTCATCGCCGCGTTCTTTTTGCTGATTTGATGCGCTATAAAGCTCAGCGTGAAA
CTGAGAGCAATGAAGCAATGCAAGAACTGGTTGATTTGAGCCAGGAACTTGGTTTTTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNB9

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3FHU5

References