Detailed information of TA system TA063337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2630723..2631357
• Replicon: chromosome
• Accession: NZ_CP015749
• Organism: Pectobacterium parmentieri strain RNS 08-42-1A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8F97_RS11990
• Protein ID: WP_033071069.1
• Coordinates: 2630953..2631357 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: A8F97_RS11985
• Protein ID: WP_012765080.1
• Coordinates: 2630723..2630953 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8F97_RS11945	2626142..2626840	+	699	WP_033071076.1	DeoR family transcriptional regulator	-
A8F97_RS11950	2627022..2627591	+	570	WP_033071075.1	hypothetical protein	-
A8F97_RS11955	2627627..2628100	+	474	WP_033071074.1	hypothetical protein	-
A8F97_RS11960	2628117..2628350	+	234	WP_033071073.1	membrane protein	-
A8F97_RS11965	2628438..2628911	+	474	WP_033071072.1	DNA repair protein RadC	-
A8F97_RS11970	2628951..2629286	+	336	WP_033071071.1	type IV toxin-antitoxin system YeeU family antitoxin	-
A8F97_RS11975	2629357..2629677	+	321	WP_033071070.1	TA system toxin CbtA family protein	-
A8F97_RS11980	2629795..2630628	+	834	WP_033071967.1	DUF4942 domain-containing protein	-
A8F97_RS11985	2630723..2630953	+	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A8F97_RS11990	2630953..2631357	+	405	WP_033071069.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A8F97_RS11995	2631747..2632191	+	445	Protein_2394	DUF4102 domain-containing protein	-
A8F97_RS12000	2632311..2632715	-	405	WP_050512634.1	hypothetical protein	-
A8F97_RS12005	2633175..2633495	-	321	WP_033071068.1	hypothetical protein	-
A8F97_RS12010	2633872..2635167	-	1296	WP_033071067.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2614646..2644473	29827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15033.24 Da
• Isoelectric Point: 7.2739

>T63337 WP_033071069.1 NZ_CP015749:2630953-2631357 [Pectobacterium parmentieri]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISAITLMELIYGAEKSVNPEKNLSVIEGFSARLEVQTYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVLGLRVENWVGQ

Nucleotide

• Length: 405 bp

>T63337 NZ_CP015749:2630953-2631357 [Pectobacterium parmentieri]
ATGCTGAAATATCTGCTCGATACCAATATCTGTATTTACACCATCAAGAACAAGCCACAGGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTGCTATCACGCTGATGGAACTGATCTATGGTGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTCTCCGTGATTGAAGGTTTTTCTGCTCGTCTGGAAGTGCAAACTTATGGCTTTGACGCTGCCGTACATACC
GGGCAAATTCGTGCCGAGCTGGCAAGACAAGGAACACCCATCGGCCCGTATGATGCCATGTTAGCGGGTCACGCGCGATC
CGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAATTTGAGCGAGTGCTTGGGCTACGTGTGGAAAACTGGGTCGGTC
AATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT63337 WP_012765080.1 NZ_CP015749:2630723-2630953 [Pectobacterium parmentieri]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT63337 NZ_CP015749:2630723-2630953 [Pectobacterium parmentieri]
ATGGAAAAAACTACAGTATTTAAAAGCAACCGTAGCCAGGCGGTACGCCTCCCGAAAGCCGTTGCACTGCCCGATGATGT
TAAGCAGGTTGATATCGTCGCGATTGGTCGCACACGAATCATCACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGATGGCGTAACGCCTGACTTTATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4